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@INPROCEEDINGS{Unverricht:841278,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Pomplun, Ekkehard
                      and Kriehuber, Ralf},
      title        = {{G}enotoxicity of {I}odine-123 labeled
                      5-{I}odo-2´-deoxyuridine in comparison to high- and
                      low-{LET} radiation},
      reportid     = {FZJ-2017-08370},
      year         = {2015},
      abstract     = {To determine the genotoxic effects after exposure to
                      Iodine-123 labeled 5-Iodo-2´-deoxyuridine (I-123-UdR) in
                      comparison to α- and γ-irradiation, micronucleus (MN)
                      induction and γ-H2AX formation were analyzed.Jurkat cells
                      were either exposed to I-123-UdR for 20 h or irradiated with
                      different doses of γ-rays (Cs-137, 0.7 Gy/min) or
                      α-particles (Am-241, 0.032 Gy/min). Cells were assayed for
                      MN formation employing automated image analysis
                      (MetaSystems, Germany). The γ-H2AX foci, as a measure of
                      DNA double-strand-breaks (dsb), were quantified by measuring
                      the mean overall signal intensity of foci per cell using
                      flow cytometry and by counting the number of individual foci
                      with a fluorescence microscope.γ-H2AX foci number per cell
                      showed a much more pronounced increase after exposure to
                      I-123-UdR per dose unit when compared to γ- and
                      α-irradiation. However, the mean intensity of total foci
                      signal per cell, as measured by flow cytometry, was very
                      similar for exposure to I-123-UdR and α-particles. Single
                      γ-H2AX foci induced by I-123-UdR appeared to be smaller
                      and/or less intense stained than those after α-irradiation
                      and resembled foci induced by γ-rays. The distribution of
                      the cellular γ-H2AX fluorescence signals showed that the
                      dose distribution of single cells was more heterogenous
                      after exposure to I-123-UdR and α-particles when compared
                      to γ-irradiation. MN induction was almost identical for all
                      three investigated radiation qualities.γ-H2AX foci are very
                      efficiently induced by I-123-UdR per unit dose when compared
                      to γ- and α-radiation, probably because almost every I-123
                      decay occurred within the DNA. The presumed complexity of
                      DNA-lesions caused by DNA-associated AEE is neither
                      reflected in size nor intensity of individual foci. The
                      microscopic quantification of γ-H2AX foci indicates that
                      I-123 induced dsb are less prone to be transferred into an
                      MN. The MN induction after exposure to I-123-UdR and
                      α-particles could be underestimated because highly damaged
                      cells within the heterogenous exposed cell population are
                      not adequately represented in the MN assay. As
                      α-particle-induced foci are aligned along the track,
                      individual foci are hard to count, we suggest that flow
                      cytometry is a more appropriate analysis tool to quantify
                      LET-dependent γ-H2AX foci induction. Funded by
                      Bundesministerium für Bildung und Forschung (BMBF), Grant
                      No.: 02NUK005A},
      month         = {May},
      date          = {2015-05-20},
      organization  = {8th International Symposium on
                       Physical, Molecular, Cellular and
                       Medical Aspects of Auger Processes,
                       Nara (Japan), 20 May 2015 - 22 May
                       2015},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841278},
}