%0 Conference Paper
%A Unverricht, Marcus
%A Giesen, Ulrich
%A Kriehuber, Ralf
%T Quantification of γ-H2AX foci following γ-rays and α-particls in Jurkat cells
%M FZJ-2017-08373
%D 2009
%X PURPOSE: Phosphorylation of histone H2AX occurs at sites flanking DNA double-strand breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We investigated whether the mean intensity measured by flow cytometry and the mean number of radiation-induced γ-H2AX foci vary as a function of radiation quality and dose. Furthermore we investigated the relation between the induction of apoptosis and the mean intensity and mean number of radiation-induced γ-H2AX foci.MATERIALS AND METHODS: Jurkat cells were irradiated with different doses of either low linear energy transfer (LET) 137Cs γ-rays or high LET 241Am α-particles. The γ-H2AX foci were detected using immunocytochemistry and quantified by measuring the mean intensity by flow cytometry and counting the number of γ-H2AX foci with a fluorescence microscope. Apoptosis 24h after irradiation was detected determining the relative DNA fragmentation rates indicative for apoptosis.RESULTS: The mean number of γ-H2AX foci increased dose dependent for both radiation qualities, but the mean intensity of γ-H2AX foci after α-radiation is much higher than after γ-radiation refered to the same dose. These data are confirmed by microscopic observations. Furthermore it seems to be that α-particles induce more apoptosis than γ-rays at the same dose and at a similar mean number of radiation-induced γ-H2AX foci.CONCLUSIONS: γ-rays and α-particles induced the phosphorylation of H2AX; the variation in the mean intensity and the mean number of radiation-induced γ-H2AX foci is dependent on radiation quality.
%B 37th Annual Meeting of the European Radiation Research Society
%C 26 Aug 2009 - 29 Aug 2009, Prague (Czech Republic)
Y2 26 Aug 2009 - 29 Aug 2009
M2 Prague, Czech Republic
%F PUB:(DE-HGF)24
%9 Poster
%U https://juser.fz-juelich.de/record/841281