TY  - CONF
AU  - Unverricht, Marcus
AU  - Giesen, Ulrich
AU  - Kriehuber, Ralf
TI  - Quantification of γ-H2AX foci following γ-rays and α-particls in Jurkat cells
M1  - FZJ-2017-08373
PY  - 2009
AB  - PURPOSE: Phosphorylation of histone H2AX occurs at sites flanking DNA double-strand breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We investigated whether the mean intensity measured by flow cytometry and the mean number of radiation-induced γ-H2AX foci vary as a function of radiation quality and dose. Furthermore we investigated the relation between the induction of apoptosis and the mean intensity and mean number of radiation-induced γ-H2AX foci.MATERIALS AND METHODS: Jurkat cells were irradiated with different doses of either low linear energy transfer (LET) 137Cs γ-rays or high LET 241Am α-particles. The γ-H2AX foci were detected using immunocytochemistry and quantified by measuring the mean intensity by flow cytometry and counting the number of γ-H2AX foci with a fluorescence microscope. Apoptosis 24h after irradiation was detected determining the relative DNA fragmentation rates indicative for apoptosis.RESULTS: The mean number of γ-H2AX foci increased dose dependent for both radiation qualities, but the mean intensity of γ-H2AX foci after α-radiation is much higher than after γ-radiation refered to the same dose. These data are confirmed by microscopic observations. Furthermore it seems to be that α-particles induce more apoptosis than γ-rays at the same dose and at a similar mean number of radiation-induced γ-H2AX foci.CONCLUSIONS: γ-rays and α-particles induced the phosphorylation of H2AX; the variation in the mean intensity and the mean number of radiation-induced γ-H2AX foci is dependent on radiation quality.
T2  - 37th Annual Meeting of the European Radiation Research Society
CY  - 26 Aug 2009 - 29 Aug 2009, Prague (Czech Republic)
Y2  - 26 Aug 2009 - 29 Aug 2009
M2  - Prague, Czech Republic
LB  - PUB:(DE-HGF)24
UR  - https://juser.fz-juelich.de/record/841281
ER  -