% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@INPROCEEDINGS{Unverricht:841281,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Kriehuber, Ralf},
      title        = {{Q}uantification of γ-{H}2{AX} foci following γ-rays and
                      α-particls in {J}urkat cells},
      reportid     = {FZJ-2017-08373},
      year         = {2009},
      abstract     = {PURPOSE: Phosphorylation of histone H2AX occurs at sites
                      flanking DNA double-strand breaks (DSBs) and can provide a
                      measure of the number of DSBs within a cell. We investigated
                      whether the mean intensity measured by flow cytometry and
                      the mean number of radiation-induced γ-H2AX foci vary as a
                      function of radiation quality and dose. Furthermore we
                      investigated the relation between the induction of apoptosis
                      and the mean intensity and mean number of radiation-induced
                      γ-H2AX foci.MATERIALS AND METHODS: Jurkat cells were
                      irradiated with different doses of either low linear energy
                      transfer (LET) 137Cs γ-rays or high LET 241Am α-particles.
                      The γ-H2AX foci were detected using immunocytochemistry and
                      quantified by measuring the mean intensity by flow cytometry
                      and counting the number of γ-H2AX foci with a fluorescence
                      microscope. Apoptosis 24h after irradiation was detected
                      determining the relative DNA fragmentation rates indicative
                      for apoptosis.RESULTS: The mean number of γ-H2AX foci
                      increased dose dependent for both radiation qualities, but
                      the mean intensity of γ-H2AX foci after α-radiation is
                      much higher than after γ-radiation refered to the same
                      dose. These data are confirmed by microscopic observations.
                      Furthermore it seems to be that α-particles induce more
                      apoptosis than γ-rays at the same dose and at a similar
                      mean number of radiation-induced γ-H2AX foci.CONCLUSIONS:
                      γ-rays and α-particles induced the phosphorylation of
                      H2AX; the variation in the mean intensity and the mean
                      number of radiation-induced γ-H2AX foci is dependent on
                      radiation quality.},
      month         = {Aug},
      date          = {2009-08-26},
      organization  = {37th Annual Meeting of the European
                       Radiation Research Society, Prague
                       (Czech Republic), 26 Aug 2009 - 29 Aug
                       2009},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841281},
}