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| Home > Publications database > Quantification of γ-H2AX foci following γ-rays and α-particls in Jurkat cells > print |
| 001 | 841281 | ||
| 005 | 20210129232007.0 | ||
| 024 | 7 | _ | |a 2128/16216 |2 Handle |
| 037 | _ | _ | |a FZJ-2017-08373 |
| 041 | _ | _ | |a English |
| 100 | 1 | _ | |a Unverricht, Marcus |0 P:(DE-Juel1)133466 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a 37th Annual Meeting of the European Radiation Research Society |g ERRS |c Prague |d 2009-08-26 - 2009-08-29 |w Czech Republic |
| 245 | _ | _ | |a Quantification of γ-H2AX foci following γ-rays and α-particls in Jurkat cells |
| 260 | _ | _ | |c 2009 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
| 336 | 7 | _ | |a INPROCEEDINGS |2 BibTeX |
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| 520 | _ | _ | |a PURPOSE: Phosphorylation of histone H2AX occurs at sites flanking DNA double-strand breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We investigated whether the mean intensity measured by flow cytometry and the mean number of radiation-induced γ-H2AX foci vary as a function of radiation quality and dose. Furthermore we investigated the relation between the induction of apoptosis and the mean intensity and mean number of radiation-induced γ-H2AX foci.MATERIALS AND METHODS: Jurkat cells were irradiated with different doses of either low linear energy transfer (LET) 137Cs γ-rays or high LET 241Am α-particles. The γ-H2AX foci were detected using immunocytochemistry and quantified by measuring the mean intensity by flow cytometry and counting the number of γ-H2AX foci with a fluorescence microscope. Apoptosis 24h after irradiation was detected determining the relative DNA fragmentation rates indicative for apoptosis.RESULTS: The mean number of γ-H2AX foci increased dose dependent for both radiation qualities, but the mean intensity of γ-H2AX foci after α-radiation is much higher than after γ-radiation refered to the same dose. These data are confirmed by microscopic observations. Furthermore it seems to be that α-particles induce more apoptosis than γ-rays at the same dose and at a similar mean number of radiation-induced γ-H2AX foci.CONCLUSIONS: γ-rays and α-particles induced the phosphorylation of H2AX; the variation in the mean intensity and the mean number of radiation-induced γ-H2AX foci is dependent on radiation quality. |
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| 700 | 1 | _ | |a Giesen, Ulrich |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Kriehuber, Ralf |0 P:(DE-Juel1)133469 |b 2 |u fzj |
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