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@ARTICLE{Minniti:841291,
author = {Minniti, Elirosa and Byl, Jo Ann W. and Riccardi, Laura and
Sissi, Claudia and Rosini, Michela and De Vivo, Marco and
Minarini, Anna and Osheroff, Neil},
title = {{N}ovel xanthone-polyamine conjugates as catalytic
inhibitors of human topoisomerase {II}α},
journal = {Bioorganic $\&$ medicinal chemistry letters},
volume = {27},
number = {20},
issn = {0960-894X},
address = {Amsterdam [u.a.]},
publisher = {Elsevier Science},
reportid = {FZJ-2017-08382},
pages = {4687 - 4693},
year = {2017},
abstract = {It has been proposed that xanthone derivatives with
anticancer potential act as topoisomerase II inhibitors
because they interfere with the ability of the enzyme to
bind its ATP cofactor. In order to further characterize
xanthone mechanism and generate compounds with potential as
anticancer drugs, we synthesized a series of derivatives in
which position 3 was substituted with different polyamine
chains. As determined by DNA relaxation and decatenation
assays, the resulting compounds are potent topoisomerase
IIα inhibitors. Although xanthone derivatives inhibit
topoisomerase IIα-catalyzed ATP hydrolysis, mechanistic
studies indicate that they do not act at the ATPase site.
Rather, they appear to function by blocking the ability of
DNA to stimulate ATP hydrolysis. On the basis of activity,
competition, and modeling studies, we propose that xanthones
interact with the DNA cleavage/ligation active site of
topoisomerase IIα and inhibit the catalytic activity of the
enzyme by interfering with the DNA strand passage step.},
cin = {IAS-5 / INM-9},
ddc = {540},
cid = {I:(DE-Juel1)IAS-5-20120330 / I:(DE-Juel1)INM-9-20140121},
pnm = {574 - Theory, modelling and simulation (POF3-574)},
pid = {G:(DE-HGF)POF3-574},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28919339},
UT = {WOS:000412863700018},
doi = {10.1016/j.bmcl.2017.09.011},
url = {https://juser.fz-juelich.de/record/841291},
}