%0 Conference Paper
%A Unverricht, Marcus
%A Giesen, Ulrich
%A Kriehuber, Ralf
%T Quantification of γ-H2AX foci following γ-rays and α-particles in Jurkat cells
%M FZJ-2017-08385
%D 2009
%X PURPOSE: Phosphorylation of histone H2AX occurs at sites flanking DNA double-strand breaks (DSBs) and can provide a measure of the number of DSBs within a cell. We investigated whether the mean intensity measured by flow cytometry and the mean number of radiation-induced γ-H2AX foci vary as a function of radiation quality and dose. Furthermore we investigated the relation between the induction of apoptosis and radiation-induced γ-H2AX foci.MATERIALS AND METHODS: Jurkat cells were irradiated with different doses of either low linear energy transfer (LET) 137Cs γ-rays or high LET 241Am α-particles. The γ-H2AX foci were detected using immunocytochemistry and quantified by measuring the mean intensity by flow cytometry and counting the number of γ-H2AX foci with a fluorescence microscope. Apoptosis 24h after irradiation was detected via Annexin-V-FITC/ PI-assay.RESULTS: For both radiation qualities, the mean number of γ-H2AX foci is increased as a function of dose and was fairly similar at identical absorbed radiation dose. Apoptosis in Jurkat cells is more efficiently induced by α-particles at similar mean numbers of γ-H2AX foci per cell. The mean γ-H2AX signal intensity of single nuclei is increased after exposure to α-particles when compared to γ-irradiation at the same absorbed radiation dose.CONCLUSIONS: The mean intensity of radiation-induced γ-H2AX foci is dependent on radiation quality in Jurkat cells.
%B 12th Annual Meeting of the German Society for Biological Radiation Research
%C 30 Sep 2009 - 2 Oct 2009, Essen (Germany)
Y2 30 Sep 2009 - 2 Oct 2009
M2 Essen, Germany
%F PUB:(DE-HGF)24
%9 Poster
%U https://juser.fz-juelich.de/record/841295