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@INPROCEEDINGS{Unverricht:841295,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Kriehuber, Ralf},
      title        = {{Q}uantification of γ-{H}2{AX} foci following γ-rays and
                      α-particles in {J}urkat cells},
      reportid     = {FZJ-2017-08385},
      year         = {2009},
      abstract     = {PURPOSE: Phosphorylation of histone H2AX occurs at sites
                      flanking DNA double-strand breaks (DSBs) and can provide a
                      measure of the number of DSBs within a cell. We investigated
                      whether the mean intensity measured by flow cytometry and
                      the mean number of radiation-induced γ-H2AX foci vary as a
                      function of radiation quality and dose. Furthermore we
                      investigated the relation between the induction of apoptosis
                      and radiation-induced γ-H2AX foci.MATERIALS AND METHODS:
                      Jurkat cells were irradiated with different doses of either
                      low linear energy transfer (LET) 137Cs γ-rays or high LET
                      241Am α-particles. The γ-H2AX foci were detected using
                      immunocytochemistry and quantified by measuring the mean
                      intensity by flow cytometry and counting the number of
                      γ-H2AX foci with a fluorescence microscope. Apoptosis 24h
                      after irradiation was detected via Annexin-V-FITC/
                      PI-assay.RESULTS: For both radiation qualities, the mean
                      number of γ-H2AX foci is increased as a function of dose
                      and was fairly similar at identical absorbed radiation dose.
                      Apoptosis in Jurkat cells is more efficiently induced by
                      α-particles at similar mean numbers of γ-H2AX foci per
                      cell. The mean γ-H2AX signal intensity of single nuclei is
                      increased after exposure to α-particles when compared to
                      γ-irradiation at the same absorbed radiation
                      dose.CONCLUSIONS: The mean intensity of radiation-induced
                      γ-H2AX foci is dependent on radiation quality in Jurkat
                      cells.},
      month         = {Sep},
      date          = {2009-09-30},
      organization  = {12th Annual Meeting of the German
                       Society for Biological Radiation
                       Research, Essen (Germany), 30 Sep 2009
                       - 2 Oct 2009},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841295},
}