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@INPROCEEDINGS{Unverricht:841299,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Kümmerle,
                      Eberhard and Pomplun, Ekkehard and Kriehuber, Ralf},
      title        = {{G}enotoxicity of {I}-123-iododeoxyuridine in vitro},
      reportid     = {FZJ-2017-08389},
      year         = {2010},
      abstract     = {OBJECTIVES: The biological effectiveness of Auger electron
                      emitters (AEE) is attributed to the numerous short-range
                      electrons released during the decay of the radionuclide.
                      Damage on cellular level depends largely on the
                      intracellular distribution of the nuclide. AEE located
                      exclusively in the cytoplasm cause e.g. low-LET type cell
                      survival in the colony-forming-assay, whereas DNA-associated
                      AEE cause high-LET type cell survival. To determine whether
                      DNA-associated AEE induce high-LET type genotoxic effects
                      micronucleus induction and γ-H2AX formation were analyzed
                      after exposure to I-123-iododeoxyuridine (I-123-UdR) in
                      comparison to high- and low-LET radiation in vitro.MATERIALS
                      AND METHODS: Human T-lymphoma Jurkat cells were either
                      exposed to I-123-UdR (0.5-50 kBq/ml) for 20 h or irradiated
                      with low-LET Cs-137 γ-rays or high-LET Am-241 α-particles.
                      Cells were assayed for micronucleus formation (Cytochalasin
                      B assay) employing automated image analysis (MetaSystems,
                      Germany). The γ-H2AX foci were quantified by measuring the
                      mean signal intensity of γ-H2AX foci per cell using flow
                      cytometry and by counting the number of γ-H2AX foci with a
                      fluorescence microscope.RESULTS: In contrast to γ- and
                      α-irradiation the numbers of γ-H2AX foci per cell showed a
                      much more pronounced increase after exposure to I-123-UdR.
                      However, the mean intensity of γ H2AX signals per cell, as
                      measured by flow cytometry, was very similar after exposure
                      to I-123-UdR and α-particles. Single γ H2AX foci induced
                      by I-123-UdR appear to be smaller and/or less intense
                      stained than those after α-irradiation and resemble γ H2AX
                      foci induced by γ-rays. Micronucleus induction was almost
                      identical for all three investigated radiation
                      qualities.CONCLUSIONS: I-123-UdR is a very potent inducer of
                      γ H2AX foci in comparison to γ- and α-radiation. Taking
                      into account the very low dose rate of I-123-UdR exposure
                      the effect is even more pronounced. Micronucleus induction
                      does not depend on radiation quality in Jurkat
                      cells.Supported by the Bundesministerium für Bildung und
                      Forschung (BMBF), Kompetenzverbund für Strahlenforschung
                      (KVSF)},
      month         = {Sep},
      date          = {2010-09-01},
      organization  = {13th Annual Meeting of the German
                       Society for Biological Radiation
                       Researc, Hamburg (Germany), 1 Sep 2010
                       - 2 Sep 2010},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841299},
}