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@INPROCEEDINGS{Unverricht:841300,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Kümmerle,
                      Eberhard and Pomplun, Ekkehard and Kriehuber, Ralf},
      title        = {{G}enotoxicity of the {A}uger electron emitter
                      {I}-123-iododeoxyuridine in vitro},
      reportid     = {FZJ-2017-08390},
      year         = {2010},
      abstract     = {OBJECTIVES: The biological effectiveness of Auger electron
                      emitters (AEE) is attributed to the numerous short-range
                      electrons released during the decay of the radionuclide.
                      Damage on cellular level depends largely on the
                      intracellular distribution of the radionuclides. AEE located
                      exclusively in the cytoplasm produce low-LET type cell
                      survival curves, whereas DNA-associated AEE cause high-LET
                      type survival curves. To determine whether AEE induce
                      high-LET type genotoxic effects micronucleus induction and
                      γ-H2AX formation were analyzed after exposure to
                      I-123-iododeoxyuridine (I-123-UdR) in comparison to high-
                      and low-LET radiation.MATERIALS AND METHODS: Human
                      T-lymphoma Jurkat cells were either exposed to I-123-UdR
                      (0.5-50 kBq/ml) for 20 h or irradiated with different doses
                      of low-LET Cs-137 γ-rays or high-LET Am-241 α-particles.
                      Cells were assayed for micronucleus formation (Cytochalasin
                      B assay) employing automated image analysis (MetaSystems,
                      Germany). The γ-H2AX foci were quantified by measuring the
                      mean signal intensity of γ-H2AX foci per cell using flow
                      cytometry and by counting the number of γ-H2AX foci with a
                      fluorescence microscope.RESULTS: In contrast to γ- and
                      α-irradiation the numbers of γ-H2AX foci per cell showed a
                      much more pronounced increase after exposure to I-123-UdR.
                      However, the mean intensity of γ H2AX signals per cell, as
                      measured by flow cytometry, was very similar for exposure to
                      I-123-UdR and α-particles. Single γ H2AX foci induced by
                      I-123-UdR appear to be smaller and/or less intense stained
                      than those after α-irradiation and resemble γ H2AX foci
                      induced by γ-rays. Micronucleus induction was almost
                      identical for all three investigated radiation
                      qualities.CONCLUSIONS: I-123-UdR is a very potent inducer of
                      γ H2AX foci in comparison to γ- and α-radiation. Taken
                      into account the very low dose rate of I-123-UdR exposure
                      the effect is even more pronounced. Micronucleus induction
                      does not depend on radiation quality in Jurkat
                      cells.Supported by the Federal Ministry of Education and
                      Research, BMBF},
      month         = {Sep},
      date          = {2010-09-05},
      organization  = {38th Annual Meeting of the European
                       Radiation Research Society, Stockholm
                       (Sweden), 5 Sep 2010 - 9 Sep 2010},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841300},
}