% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@INPROCEEDINGS{Unverricht:841301,
      author       = {Unverricht, Marcus and Giesen, Ulrich and Kriehuber, Ralf},
      title        = {{Q}uantification of γ-{H}2{AX} foci after exposure to
                      {I}-123-iododeoxyuridine in comparison to γ- and
                      α-irradiation},
      reportid     = {FZJ-2017-08391},
      year         = {2011},
      abstract     = {Introduction: Phosphorylation of histone H2AX occurs at
                      sites flanking DNA double-strand breaks (DSBs) and can
                      provide an indirect measure for the number of DSBs within a
                      cell. Recent publications suggest LET-dependent differences
                      in the intensity and size of γ-H2AX foci. To determine
                      whether γ-H2AX foci caused by DNA-associated Auger electron
                      emitters (AEE) induce high-LET type γ-H2AX foci we
                      investigated the mean intensity as well as the mean number
                      of γ-H2AX foci after exposure to I-123, high- and low-LET
                      radiation.Methods: Human T-lymphoma Jurkat cells were either
                      exposed to I-123-iododeoxyuridine (I-123-UdR; 2-200 kBq per
                      10E6 cells) for 20 h or irradiated with different doses of
                      low-LET Cs-137 γ-rays respectively high-LET Am-241
                      α-particles. The γ-H2AX foci were quantified by measuring
                      the mean signal intensity using flow cytometry and by
                      counting the number of γ-H2AX foci microscopically by eye.
                      Co-localization experiments were performed with the
                      DNA-repair associated protein 53BP1 employing confocal
                      microscopy.Results: The mean numbers of γ-H2AX foci per
                      cell showed a much more pronounced increase after exposure
                      to I-123 when compared to γ- and α-irradiation. However,
                      the mean intensity of γ H2AX signals per cell nucleus, was
                      very similar after I-123 and α-particle exposure. The
                      individual γ H2AX foci induced by I-123 resemble γ H2AX
                      foci induced by γ-rays and appear to be smaller, more
                      distinct and/or less intense stained than those after
                      α-irradiation. 53BP1 foci do not always co-localize with
                      γ-H2AX foci. Conclusions: The presumed complexity of the
                      DNA-lesion caused by DNA-associated AEE is not reflected in
                      the size and the intensity of γ-H2AX foci.Funded by
                      Bundesministerium für Bildung und Forschung (BMBF), Project
                      No.: 02NUK005A},
      month         = {Aug},
      date          = {2011-08-24},
      organization  = {7th International Symposium on
                       Physical, Molecular, Cellular and
                       Medical Aspects of Auger Processes
                       2011, Jülich (Germany), 24 Aug 2011 -
                       26 Aug 2011},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/841301},
}