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| Home > Publications database > Quantification of γ-H2AX foci after exposure to I-123-iododeoxyuridine in comparison to γ- and α-irradiation > print |
| Home > Publications database > Quantification of γ-H2AX foci after exposure to I-123-iododeoxyuridine in comparison to γ- and α-irradiation > print |
| 001 | 841301 | ||
| 005 | 20210129232010.0 | ||
| 024 | 7 | _ | |a 2128/16247 |2 Handle |
| 037 | _ | _ | |a FZJ-2017-08391 |
| 041 | _ | _ | |a English |
| 100 | 1 | _ | |a Unverricht, Marcus |0 P:(DE-Juel1)133466 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a 7th International Symposium on Physical, Molecular, Cellular and Medical Aspects of Auger Processes 2011 |c Jülich |d 2011-08-24 - 2011-08-26 |w Germany |
| 245 | _ | _ | |a Quantification of γ-H2AX foci after exposure to I-123-iododeoxyuridine in comparison to γ- and α-irradiation |
| 260 | _ | _ | |c 2011 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
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| 520 | _ | _ | |a Introduction: Phosphorylation of histone H2AX occurs at sites flanking DNA double-strand breaks (DSBs) and can provide an indirect measure for the number of DSBs within a cell. Recent publications suggest LET-dependent differences in the intensity and size of γ-H2AX foci. To determine whether γ-H2AX foci caused by DNA-associated Auger electron emitters (AEE) induce high-LET type γ-H2AX foci we investigated the mean intensity as well as the mean number of γ-H2AX foci after exposure to I-123, high- and low-LET radiation.Methods: Human T-lymphoma Jurkat cells were either exposed to I-123-iododeoxyuridine (I-123-UdR; 2-200 kBq per 10E6 cells) for 20 h or irradiated with different doses of low-LET Cs-137 γ-rays respectively high-LET Am-241 α-particles. The γ-H2AX foci were quantified by measuring the mean signal intensity using flow cytometry and by counting the number of γ-H2AX foci microscopically by eye. Co-localization experiments were performed with the DNA-repair associated protein 53BP1 employing confocal microscopy.Results: The mean numbers of γ-H2AX foci per cell showed a much more pronounced increase after exposure to I-123 when compared to γ- and α-irradiation. However, the mean intensity of γ H2AX signals per cell nucleus, was very similar after I-123 and α-particle exposure. The individual γ H2AX foci induced by I-123 resemble γ H2AX foci induced by γ-rays and appear to be smaller, more distinct and/or less intense stained than those after α-irradiation. 53BP1 foci do not always co-localize with γ-H2AX foci. Conclusions: The presumed complexity of the DNA-lesion caused by DNA-associated AEE is not reflected in the size and the intensity of γ-H2AX foci.Funded by Bundesministerium für Bildung und Forschung (BMBF), Project No.: 02NUK005A |
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| 700 | 1 | _ | |a Giesen, Ulrich |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Kriehuber, Ralf |0 P:(DE-Juel1)133469 |b 2 |u fzj |
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