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@INPROCEEDINGS{Unverricht:841302,
      author       = {Unverricht, Marcus and Boldt, Sonja and Giesen, Ulrich and
                      Pomplun, Ekkehard and Wolkenhauer, Olaf and Kriehuber, Ralf},
      title        = {{W}hole genome expression analysis in human {J}urkat cells
                      after exposure to {I}-123-iododeoxyuridine, γ-rays and
                      α-particles},
      reportid     = {FZJ-2017-08392},
      year         = {2011},
      abstract     = {Introduction: In order to develop a gene expression
                      profile-based method for biodosimetry purposes we used the
                      human p53-deficient T-lymphoma Jurkat cell line to study
                      whether gene signatures exist allowing the discrimination of
                      radiation quality.Methods: Equi-effect doses, i.e. radiation
                      doses and exposure conditions causing the same biological
                      effect level, were determined with regard to micronucleus
                      formation, γ-H2AX foci intensity and apoptosis induction
                      for the radiation qualities of γ-rays (Cs-137) and
                      α-particles (Am-241) as well as for the Auger electron
                      emitter I-123. Prior to the DNA-microarray based gene
                      expression experiments, Jurkat cells were either irradiated
                      with 0.8 and 5 Gy γ-rays, respectively with 0.1 and 0.5 Gy
                      α-particles or were exposed to 4 - 200 kBq
                      I-123-iododeoxyuridine (I-123-UdR) per 10E6 cells. I-123-UdR
                      was incorporated into the DNA for 20 h. After quantification
                      of cellular uptake and calculation of accumulated decays the
                      absorbed radiation dose was assessed based on the 3-D
                      geometry of the cells. RNA-isolation was performed 6 h and
                      24 h post-exposure. Whole human genome DNA-microarrays
                      (Agilent) were processed and expression profiles were
                      analyzed. Genes showing significant expression changes after
                      irradiation were identified by one-way ANOVA and Tukey-HSD
                      post-hoc testing. The biological functions of significantly
                      regulated genes were further investigated.Results:
                      Preliminary results of the gene expression analysis indicate
                      that the expression of more and different genes is
                      significantly altered after exposure to I-123-UdR and
                      α-particles when compared to γ-irradiation. The functional
                      analysis of significantly changed genes reveals that
                      apoptosis relevant genes are enriched after exposure to
                      I-123-UdR and α-particles in comparison to γ-irradiation.
                      Conclusions: I-123-UdR and α-particles induce pronounced
                      alterations in gene expression when compared to γ-rays.
                      Changes in the gene expression of p53-dependent
                      apoptosis-related genes were observed suggesting
                      p53-independent back-up pathways for apoptosis signalling in
                      Jurkat cells.Funded by Bundesministerium für Bildung und
                      Forschung (BMBF), Project No.: 02NUK005A and 02NUK005D},
      month         = {Aug},
      date          = {2011-08-24},
      organization  = {7th International Symposium on
                       Physical, Molecular, Cellular and
                       Medical Aspects of Auger Processes
                       2011, Jülich (Germany), 24 Aug 2011 -
                       26 Aug 2011},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841302},
}