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| Home > Publications database > Whole genome expression analysis in human Jurkat cells after exposure to I-123-iododeoxyuridine, γ-rays and α-particles > print |
| 001 | 841302 | ||
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| 100 | 1 | _ | |a Unverricht, Marcus |0 P:(DE-Juel1)133466 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a 7th International Symposium on Physical, Molecular, Cellular and Medical Aspects of Auger Processes 2011 |c Jülich |d 2011-08-24 - 2011-08-26 |w Germany |
| 245 | _ | _ | |a Whole genome expression analysis in human Jurkat cells after exposure to I-123-iododeoxyuridine, γ-rays and α-particles |
| 260 | _ | _ | |c 2011 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
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| 520 | _ | _ | |a Introduction: In order to develop a gene expression profile-based method for biodosimetry purposes we used the human p53-deficient T-lymphoma Jurkat cell line to study whether gene signatures exist allowing the discrimination of radiation quality.Methods: Equi-effect doses, i.e. radiation doses and exposure conditions causing the same biological effect level, were determined with regard to micronucleus formation, γ-H2AX foci intensity and apoptosis induction for the radiation qualities of γ-rays (Cs-137) and α-particles (Am-241) as well as for the Auger electron emitter I-123. Prior to the DNA-microarray based gene expression experiments, Jurkat cells were either irradiated with 0.8 and 5 Gy γ-rays, respectively with 0.1 and 0.5 Gy α-particles or were exposed to 4 - 200 kBq I-123-iododeoxyuridine (I-123-UdR) per 10E6 cells. I-123-UdR was incorporated into the DNA for 20 h. After quantification of cellular uptake and calculation of accumulated decays the absorbed radiation dose was assessed based on the 3-D geometry of the cells. RNA-isolation was performed 6 h and 24 h post-exposure. Whole human genome DNA-microarrays (Agilent) were processed and expression profiles were analyzed. Genes showing significant expression changes after irradiation were identified by one-way ANOVA and Tukey-HSD post-hoc testing. The biological functions of significantly regulated genes were further investigated.Results: Preliminary results of the gene expression analysis indicate that the expression of more and different genes is significantly altered after exposure to I-123-UdR and α-particles when compared to γ-irradiation. The functional analysis of significantly changed genes reveals that apoptosis relevant genes are enriched after exposure to I-123-UdR and α-particles in comparison to γ-irradiation. Conclusions: I-123-UdR and α-particles induce pronounced alterations in gene expression when compared to γ-rays. Changes in the gene expression of p53-dependent apoptosis-related genes were observed suggesting p53-independent back-up pathways for apoptosis signalling in Jurkat cells.Funded by Bundesministerium für Bildung und Forschung (BMBF), Project No.: 02NUK005A and 02NUK005D |
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