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@INPROCEEDINGS{Unverricht:841303,
      author       = {Unverricht, Marcus and Boldt, Sonja and Giesen, Ulrich and
                      Pomplun, Ekkehard and Wolkenhauer, Olaf and Kriehuber, Ralf},
      title        = {{WHOLE} {GENOME} {EXPRESSION} {ANALYSIS} {IN} {HUMAN}
                      {JURKAT} {CELLS} {AFTER} {EXPOSURE} {TO}
                      {I}-123-{IODODEOXYURIDINE}, γ-{RAYS} {AND} α-{PARTICLES}},
      reportid     = {FZJ-2017-08393},
      year         = {2011},
      abstract     = {Introduction: In order to develop a gene expression
                      profile-based method for biodosimetry purposes we used the
                      human p53-deficient T-lymphoma Jurkat cell line to study
                      whether gene signatures exist allowing the discrimination of
                      radiation quality as well.Methods: Equi-effect doses, i.e.
                      radiation doses and exposure conditions causing the same
                      biological effect level, were determined with regard to
                      micronucleus formation, γ-H2AX foci intensity and apoptosis
                      induction for the radiation qualities of γ-rays (Cs-137)
                      and α-particles (Am-241) as well as for the Auger electron
                      emitter I-123. Prior to the DNA-microarray based gene
                      expression experiments, Jurkat cells were either irradiated
                      with 0.8 and 5 Gy γ-rays, respectively with 0.1 and 0.5 Gy
                      α-particles or were exposed to 4 - 200 kBq
                      I-123-iododeoxyuridine (I-123-UdR) per 10E6 cells. I-123-UdR
                      was incorporated into the DNA of synchronized cells for 20
                      h. After quantification of the cellular uptake the
                      accumulated decays were calculated and the absorbed
                      radiation dose was assessed after 3-D geometry analysis of
                      the cells. RNA-isolation was performed always 6 h
                      post-exposure. Whole human genome DNA-microarrays (Agilent)
                      were processed and expression profiles were analyzed. Genes
                      showing significant expression changes after irradiation
                      were identified by one-way ANOVA and Tukey-HSD post-hoc
                      testing. The biological functions of significantly regulated
                      genes were further investigated.Results: Preliminary results
                      of the gene expression analysis after exposure to the three
                      investigated radiation qualities indicate that the
                      expression of more and different genes is significantly
                      altered after exposure to I-123-UdR when compared to γ- and
                      α-irradiation. The functional analysis of significantly
                      changed genes reveals that apoptosis relevant genes are
                      enriched after exposure to I-123-UdR in comparison to γ-
                      and α-irradiation. Conclusions: I-123-UdR induces
                      pronounced alterations in gene expression when compared to
                      γ-rays and α-particles. Changes in the gene expression of
                      p53-dependent apoptosis-related genes were observed after
                      I-123-UdR exposure suggesting p53-independent back-up
                      pathways for apoptosis signalling in Jurkat cells.Funded by
                      Kompetenzverbund Strahlenforschung (KVSF), Bundesministerium
                      für Bildung und Forschung (BMBF), Project No.: 02NUK005A
                      and 02NUK005D},
      month         = {Sep},
      date          = {2011-09-13},
      organization  = {14. Jahrestagung der Gesellschaft für
                       biologische Strahlenforschung,
                       Rheinbach (Germany), 13 Sep 2011 - 16
                       Sep 2011},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/841303},
}