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| Hauptseite > Publikationsdatenbank > Cytotoxicity and induction of cell cycle arrest in SCL-II cells after transfection with I-125 labeled Triplex-Forming Oligonucleotides > print |
| Hauptseite > Publikationsdatenbank > Cytotoxicity and induction of cell cycle arrest in SCL-II cells after transfection with I-125 labeled Triplex-Forming Oligonucleotides > print |
| 001 | 841317 | ||
| 005 | 20210129232013.0 | ||
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| 037 | _ | _ | |a FZJ-2017-08406 |
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| 100 | 1 | _ | |a Dahmen, Volker |0 P:(DE-Juel1)133468 |b 0 |e Corresponding author |u fzj |
| 111 | 2 | _ | |a 15. Jahrestagung der Gesellschaft für biologische Strahlenforschung |g GBS |c München |d 2012-09-17 - 2012-09-20 |w Germany |
| 245 | _ | _ | |a Cytotoxicity and induction of cell cycle arrest in SCL-II cells after transfection with I-125 labeled Triplex-Forming Oligonucleotides |
| 260 | _ | _ | |c 2012 |
| 336 | 7 | _ | |a Conference Paper |0 33 |2 EndNote |
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| 520 | _ | _ | |a Introduction: Triplex-forming oligonucleotides (TFOs) are able to bind DNA in a sequence specific manner and are a promising tool to manipulate genes or gene regulatory units in a cellular environment. TFOs posses also a therapeutic potential e.g. as a carrier molecule for Alpha- or Auger-Electron-Emitter (AEE) to target specific DNA sequences in tumor cells. We established a method for the effective labeling of TFOs with the AEE iodine-125 (I-125) and studied the influence of labeled TFOs in transfected SCL-II cells with regard to cell survival, appearance of DNA Double-Strand-Breaks (DSB) and the induction of cell cycle arrest.Methods: The TFOs employed were a single binding site TFO, specific for GAPDH and two multi-binding TFOs with several thousand binding sites in the human genome. TFO labeling with I-125 was performed using the primer extension method. Cell survival and DNA DSB frequency in I-125-TFO transfected SCL-II cells were analyzed with the Colony-Forming-Assay and the 53BP1 Assay. Analysis of cell cycle was done after 7-AAD staining by flowcytometry.Results: All three tested TFOs induced a pronounced cytotoxic effect on SCL-II cells. The broadest effect was found for the TFO employing the highest target number in the genome. Furthermore an increased frequency of DSB and a significant cell cycle arrest in G2/M phase 8 h post-transfection could be detected.Conclusion: I-125 labeled TFOs with multiple binding sites as well as single binding TFOs do induce a pronounced cytotoxic effect and increase the DSB frequency in SCL-II cells. Additionally there is strong evidence that I-125-labeled TFOs can influence the cell cycle course in transfected SCL-I cells. Funded by the Bundesministerium für Bildung und Forschung (BMBF), Kompetenzverbund Strahlenforschung (KVSF), Project No.: 02NUK005A |
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| 700 | 1 | _ | |a Kriehuber, Ralf |0 P:(DE-Juel1)133469 |b 1 |u fzj |
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