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@INPROCEEDINGS{Kriehuber:841531,
      author       = {Kriehuber, Ralf and von Ameln, Marcel and Bahn, S. and
                      Pomplun, Ekkehard},
      title        = {{I}ncreased micronucleus induction in 170{T}m-irradiated
                      nanogold-labeled {SCL} {II}-cells},
      reportid     = {FZJ-2017-08575},
      year         = {2008},
      abstract     = {Photoelectric absorption of photons with energies slightly
                      above the K shell binding energy of an appropriate element
                      can result in the emission of a shower of low-energy
                      Auger-electrons. It is well known that those electrons
                      released by Auger-electron-emitting radionuclides located in
                      the immediate vicinity of the DNA cause high-LET-type damage
                      and induce an enhanced relative biological effectiveness
                      when compared to low-LET radiation. Therefore, an enhanced
                      biological effectiveness is expected after photon activation
                      as well. To proof if photoelectric absorption leads to an
                      increased cellular radiotoxicity we investi¬gated in SCL
                      II-cells whether photon activation of intracellular located
                      nano-sized gold par¬ticles is feasible to enhance cyto- and
                      genotoxic effects in vitro.SCL II-cells were transfected
                      with colloidal nano-sized gold particles (40 nm) and
                      gold-labeled DNA-triplex-forming-oligonucleotides and
                      irradiated with a suitable 170Tm source (micro seeds).
                      Genotoxicity was assessed using the Micronucleus-Assay and
                      cytotoxicity was in-vestigated using the
                      Colony-Forming-Assay.Preliminary results indicate that
                      Nanogold-labeled SCL II-cells show a 2-fold increase in
                      micronucleus formation when compared to irradiated
                      non-labeled cells. Non-irradiated Nanogold-labeled SCL
                      II-cells showed the same background level of micronucleated
                      cells as non-labeled SCLII-cells. The mitotic activity was
                      neither disturbed by the gold-labeling nor the transfection
                      procedure. Cytotoxic effects are less prominent but still
                      need further investi¬gation. Photon activation might be a
                      promising approach to increase the biological effectiveness
                      of low-LET-radiation and might be of great value for new
                      brachytherapy strategies.This work is financially supported
                      by STEP Pockau GmbH},
      month         = {Sep},
      date          = {2008-09-01},
      organization  = {36th Annual Meeting of the European
                       Radiation Research Society, Tours
                       (France), 1 Sep 2008 - 4 Sep 2008},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)6},
      url          = {https://juser.fz-juelich.de/record/841531},
}