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@ARTICLE{Moudkov:842144,
      author       = {Moudříková, Šárka and Sadowsky, Andres and Metzger,
                      Sabine and Nedbal, Ladislav and Mettler-Altmann, Tabea and
                      Mojzeš, Peter},
      title        = {{Q}uantification of {P}olyphosphate in {M}icroalgae by
                      {R}aman {M}icroscopy and by a {R}eference {E}nzymatic
                      {A}ssay},
      journal      = {Analytical chemistry},
      volume       = {89},
      number       = {22},
      issn         = {1520-6882},
      address      = {Columbus, Ohio},
      publisher    = {American Chemical Society},
      reportid     = {FZJ-2018-00423},
      pages        = {12006 - 12013},
      year         = {2017},
      abstract     = {Polyphosphates have occurred in living cells early in
                      evolution and microalgae also contain these important
                      polymers in their cells. Progress in research of
                      polyphosphate metabolism of these ecologically as well
                      asbiotechnologically important microorganisms is hampered by
                      the lack of rapid quantification methods. Experiments with
                      the green alga model Chlorella vulgaris presented here
                      compared polyphosphate extraction in water,
                      methanol-chloroform, and phenol-chloroform followed by
                      polyphosphate purification by binding to silica columns or
                      ethanol precipitation. The phenol-chloroform extraction of
                      C. vulgaris followed by ethanol precipitation of
                      polyphosphate was shown to be superior to the other tested
                      method variants. Recovery of added polyphosphate standard to
                      algal biomass showed that the method is accurate. Using this
                      biochemical assay as a validated reference, we show that
                      2-dimensional, confocal Raman microscopy can serve as a
                      linear proxy for polyphosphate in C. vulgaris with R2 up to
                      0.956. With this, polyphosphate quantificationcan be
                      shortened by use of Raman microscopy from days to hours and,
                      additionally, information about intracellular distribution
                      of polyphosphate and heterogeneity among individual cells in
                      algal culture can be obtained. This offers new insights into
                      the dynamics and role of these polymers crucial for
                      phosphorus uptake and storage. This analytical capability is
                      of particular practical importance because algae aid
                      phosphorus sequestration from wastewater and the thus
                      enriched biomass may serve as organic fertilizer. Both these
                      applications have a strong potential in a future
                      sustainable, circular bioeconomy.},
      cin          = {IBG-2},
      ddc          = {540},
      cid          = {I:(DE-Juel1)IBG-2-20101118},
      pnm          = {582 - Plant Science (POF3-582) / AF AlgalFertilizer -
                      AlgalFertilizer (20172303)},
      pid          = {G:(DE-HGF)POF3-582 / G:(BioSC)20172303},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29099580},
      UT           = {WOS:000416498100019},
      doi          = {10.1021/acs.analchem.7b02393},
      url          = {https://juser.fz-juelich.de/record/842144},
}