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@INBOOK{Bornscheuer:842291,
      author       = {Fulton, Alexander and Hayes, Marc R. and Schwaneberg,
                      Ulrich and Pietruszka, Jörg and Jaeger, Karl-Erich},
      editor       = {Bornscheuer, Uwe T. and Höhne, Matthias},
      title        = {{H}igh-{T}hroughput {S}creening {A}ssays for {L}ipolytic
                      {E}nzymes},
      volume       = {1685},
      address      = {New York, NY},
      publisher    = {Springer New York},
      reportid     = {FZJ-2018-00538},
      isbn         = {978-1-4939-7364-4 (print)},
      series       = {Methods in Molecular Biology},
      pages        = {209 - 231},
      year         = {2018},
      comment      = {Protein Engineering / Bornscheuer, Uwe T. (Editor) ; New
                      York, NY : Springer New York, 2018, Chapter 12 ; ISSN:
                      1064-3745=1940-6029 ; ISBN:
                      978-1-4939-7364-4=978-1-4939-7366-8 ;
                      doi:10.1007/978-1-4939-7366-8},
      booktitle     = {Protein Engineering / Bornscheuer, Uwe
                       T. (Editor) ; New York, NY : Springer
                       New York, 2018, Chapter 12 ; ISSN:
                       1064-3745=1940-6029 ; ISBN:
                       978-1-4939-7364-4=978-1-4939-7366-8 ;
                       doi:10.1007/978-1-4939-7366-8},
      abstract     = {Screening is defined as the identification of hits within a
                      large library of variants of an enzyme or protein with a
                      predefined property. In theory, each variant present in the
                      respective library needs to be assayed; however, to save
                      time and consumables, many screening regimes involve a
                      primary round to identify clones producing active enzymes.
                      Such primary or prescreenings for lipolytic enzyme activity
                      are often carried out on agar plates containing pH
                      indicators or substrates as triolein or tributyrin.
                      Subsequently, high-throughput screening assays are usually
                      performed in microtiter plate (MTP) format using chromogenic
                      or fluorogenic substrates and, if available, automated
                      liquid handling robotics. Here, we describe different assay
                      systems to determine the activity and enantioselectivity of
                      lipases and esterases as well as the synthesis of several
                      substrates. We also report on the construction of a complete
                      site saturation library derived from lipase A of Bacillus
                      subtilis and its testing for detergent tolerance. This
                      approach allows for the identification of amino acids
                      affecting sensitivity or resistance against different
                      detergents.},
      cin          = {IMET / IBOC / IBG-1},
      ddc          = {570},
      cid          = {I:(DE-Juel1)IMET-20090612 / I:(DE-Juel1)IBOC-20090406 /
                      I:(DE-Juel1)IBG-1-20101118},
      pnm          = {581 - Biotechnology (POF3-581)},
      pid          = {G:(DE-HGF)POF3-581},
      typ          = {PUB:(DE-HGF)7},
      pubmed       = {pmid:29086311},
      UT           = {WOS:000684272200013},
      doi          = {10.1007/978-1-4939-7366-8_12},
      url          = {https://juser.fz-juelich.de/record/842291},
}