TY  - JOUR
AU  - Sonnack, Laura
AU  - Klawonn, Thorsten
AU  - Kriehuber, Ralf
AU  - Hollert, Henner
AU  - Schäfers, Christoph
AU  - Fenske, Martina
TI  - Comparative analysis of the transcriptome responses of zebrafish embryos after exposure to low concentrations of cadmium, cobalt and copper
JO  - Comparative biochemistry and physiology / D
VL  - 25
SN  - 1744-117X
CY  - New York, NY [u.a.]
PB  - Elsevier
M1  - FZJ-2018-00773
SP  - 99 - 108
PY  - 2018
AB  - Metal toxicity is a global environmental challenge. Fish are particularly prone to metal exposure, which can be lethal or cause sublethal physiological impairments. The objective ofthis study was to investigate how adverse effects of chronic exposure to non-toxic levels of essential and non-essential metals in early life stage zebrafish may be explained by changes inthe transcriptome. We therefore studied the effects of three different metals at low concentrations in zebrafish embryos by transcriptomics analysis. The study design comparedexposure effects caused by different metals at different developmental stages (pre-hatch and post-hatch). Wild-type embryos were exposed to solutions of low concentrations of copper(CuSO4), cadmium (CdCl2) and cobalt (CoSO4) until 96 h post-fertilization (hpf) and microarray experiments were carried out to determine transcriptome profiles at 48 and 96 hpf.We found that the toxic metal cadmium affected the expression of more genes at 96 hpf than 48 hpf. The opposite effect was observed for the essential metals cobalt and copper, whichalso showed enrichment of different GO terms. Genes involved in neuromast and motor neuron development were significantly enriched, agreeing with our previous results showingmotor neuron and neuromast damage in the embryos. Our data provide evidence that the response of the transcriptome of fish embryos to metal exposure differs for essential and nonessential metals.
LB  - PUB:(DE-HGF)16
C6  - pmid:29287281
UR  - <Go to ISI:>//WOS:000426535700011
DO  - DOI:10.1016/j.cbd.2017.12.001
UR  - https://juser.fz-juelich.de/record/842554
ER  -