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@INPROCEEDINGS{Schmitz:842641,
      author       = {Schmitz, Sabine and Brzozowska, Kinga and Pinkawa, Michael
                      and Eble, Michael and Kriehuber, Ralf},
      title        = {{C}hromosomal radiosensitivity of lymphocytes of prostate
                      cancer patients and healthy donors analyzed by {FISH}},
      reportid     = {FZJ-2018-00848},
      year         = {2011},
      abstract     = {Abstract: Background: It is known that about 10 $\%$ of
                      cancer patients show severe clinical side effects during and
                      after radiotherapy due to enhanced sensitivity to ionizing
                      radiation. Identification of those radiosensitive
                      individuals by an in vitro assay before onset of treatment
                      would be of great impact for successful radiotherapy.In this
                      study we compared the radiosensitivity of the chromosomes 2,
                      11 and 17 in prostate cancer patients with and without
                      severe side effects after radiotherapy and in age-matched
                      healthy donors (control cohort). The chromosomal
                      radiosensitivity of peripheral blood lymphocytes (PBL) of
                      radiotherapy patients was used as predictive parameter for
                      clinical side effects.Material und Methods: Each cohort
                      consisted of at least 10 donors. PBL were irradiated ex vivo
                      with 0.5, 1 und 2 Gy (Cs-137 -rays) in the G0-Phase of
                      the cell cycle. We analyzed the radiosensitivity of the
                      chromosomes 2, 11 and 17 by scoring of 100 FISH painted
                      metaphases for each dose point. Statistical analyses were
                      performed by non-parametric Mann-Whitney test, by test of
                      variances (ANOVA) and Chi-square goodness-of-fit test at a
                      significance level of 0.05. Results: Analysis of the overall
                      aberration yield revealed no significant differences between
                      any donor groups. However, variance analyses showed
                      significant differences between the patient’s cohort and
                      healthy donors for chromosomes 11 and 17 for all doses
                      analyzed. In contrast, this was not true for chromosome 2.
                      Furthermore, good correlations between chromosomes sizes
                      (DNA content) and aberration yield were found.Conclusion:
                      The cohort of prostate cancer patients can be distinguished
                      from healthy donors due to variances of the aberration
                      yields of the chromosomes 11 and 17. These chromosomes might
                      be potential cytogenetic biomarkers for prostate cancer
                      patients in clinical studies.Funded by Dr.
                      Erich-Schmitt-Foundation},
      month         = {Sep},
      date          = {2011-09-13},
      organization  = {14. Jahrestagung der Gesellschaft für
                       biologische Strahlenforschung,
                       Rheinbach (Germany), 13 Sep 2011 - 16
                       Sep 2011},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/842641},
}