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@INPROCEEDINGS{Brzozowska:842644,
      author       = {Brzozowska, Kinga and Pomplun, Ekkehard and Kriehuber,
                      Ralf},
      title        = {{C}hromosome aberrations in human peripheral blood
                      lymphocytes and h{TERT}-{RPE}1 cell line after exposure to
                      the {A}uger electron emitter {I}-125},
      reportid     = {FZJ-2018-00851},
      year         = {2011},
      abstract     = {Introduction: Auger electron emitters (AEE) have a high
                      potential for targeted tumour therapy due to their strongly
                      localised energy deposition. DNA-associated AEE induce
                      cellular damage leading to high-LET-type cell survival
                      curves and possess enhanced relative biological
                      effectiveness. They are presumed to cause complex DNA
                      lesions as well. To elucidate the genotoxic potential of
                      DNA-associated AEE, chromosomal/chromatid aberrations were
                      analyzed in Iodine-125-deoxyuridine (I-125-UdR) exposed
                      human peripheral blood lymphocytes (PBL) and in human
                      telomerase-immortalized retinal pigment epithelial cells
                      (hTERT-RPE1).Methods: For each donor, PBL were incubated
                      with I-125-UdR for 5 h (2.5 and 5 kBq/ml). The culture was
                      fixed for aberrations at 72 h post-stimulation. hTERT-RPE1
                      cells were exposed to I-125-UdR activities ranging from 0.5
                      – 4 kBq/ml. The cells were harvested for aberrations at 26
                      h after initiation of the culture. All slides were stained
                      with 10 $\%$ Giemsa. For each dose point 100 metaphases were
                      analysed. For both PBL and hTERT-RPE1 cells, cell cycle
                      analysis using EdU Flow Cytometry Assay Kits (Invitrogen)
                      was performed.Results: Our preliminary data show that
                      I-125-UdR primarily induce chromatid-type aberrations. An
                      enhanced level of aberrations was observed at already 100
                      accumulated decays per cell, whereof one decay per cell per
                      20 min was calculated. The cell cycle of both PBL and
                      hTERT-RPE1 cell line are delayed due to incorporation of
                      I-125-UdR in the DNA, when compared to control cells.
                      Conclusions: I-125-UdR posses a strong genotoxic capacity in
                      PBL and hTERT-RPE1 cells even at very low numbers of
                      accumulated decays. Supported by the Bundesministerium für
                      Bildung und Forschung (BMBF), Kompetenzverbund für
                      Strahlenforschung (KVSF), Project No.: 02NUK005A},
      month         = {Aug},
      date          = {2011-08-28},
      organization  = {14th International Congress of
                       Radiation Research, Warsaw (Poland), 28
                       Aug 2011 - 1 Sep 2011},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/842644},
}