000842649 001__ 842649
000842649 005__ 20210129232419.0
000842649 0247_ $$2Handle$$a2128/16854
000842649 037__ $$aFZJ-2018-00856
000842649 041__ $$aEnglish
000842649 1001_ $$0P:(DE-HGF)0$$aBrzozowska, Kinga$$b0$$eCorresponding author
000842649 1112_ $$a37th Annual Meeting of the European Radiation Research Society$$cPrague$$d2009-08-26 - 2009-08-29$$gERRS$$wCzech Republic
000842649 245__ $$aComparison of individual radiosensitivity of peripheral blood lymphocytes from prostate cancer patients and healthy donors
000842649 260__ $$c2009
000842649 3367_ $$033$$2EndNote$$aConference Paper
000842649 3367_ $$2BibTeX$$aINPROCEEDINGS
000842649 3367_ $$2DRIVER$$aconferenceObject
000842649 3367_ $$2ORCID$$aCONFERENCE_POSTER
000842649 3367_ $$2DataCite$$aOutput Types/Conference Poster
000842649 3367_ $$0PUB:(DE-HGF)24$$2PUB:(DE-HGF)$$aPoster$$bposter$$mposter$$s1517208898_23350$$xAfter Call
000842649 520__ $$aIntroduction: There is some evidence that approximately 10% of the population show an enhanced intrinsic radiosensitivity of normal tissue and hence have a higher risk for developing side-effects during or after radiotherapy. For that reason there is a need for a fast and robust test to assess normal tissue response to radiotherapy. DNA repair deficiency and altered apoptosis characteristics are discussed as promising markers of radiosensitivity. The aim of our study was to find out whether peripheral blood lymphocytes (PBLs) from cancer patients with strong clinical side effects following radiotherapy as assessed clinically on the basis of the RTOG/EORTC scale, show enhanced rates of in vitro radiation-induced double strand breaks (dsb), decreased DNA repair capacity and altered induction of apoptosis when compared to lymphocytes from patients without side effects and healthy age–matched donors. Materials and methods: The study included 20 prostate cancer patients without and 20 patients with acute side-effects during and after radiothrapy, as well as 20 healthy age-matched donors. From each donor, blood samples were collected, exposed to a radiation dose of 0.5 Gy or 1 Gy γ –rays and analysed for the following biological endpoints: the initial level of dsb and the repair kinetics (γ–H2AX–assay), apoptosis (Annexin V/PI–assay) and the induction of chromatid–type chromosomal aberrations (G2–assay). Additionally FISH analysis (G0-aberrations) were performed for some patients of each group. Results: The maximum number of γ–H2AX foci occurred at 30 min after exposure without significant differences in numbers of foci between both groups of patients and healthy donors. The frequency of spontaneous and radiation-induced apoptoses was determined 30 min, 5 h and 24 h post- irradiation and showed a significant increase of early apoptosis 24 h post-irradiation for both patients and healthy donors. The rate of necrosis had a tendency to increase 24 h post-irradiation in patients with strong clinical side effects. The highest level of aberrations was observed for patients with strong clinical side effects in comparison to patients without any side effects and healthy donors.
000842649 536__ $$0G:(DE-HGF)POF3-899$$a899 - ohne Topic (POF3-899)$$cPOF3-899$$fPOF III$$x0
000842649 65027 $$0V:(DE-MLZ)SciArea-160$$2V:(DE-HGF)$$aBiology$$x0
000842649 7001_ $$0P:(DE-HGF)0$$aWojcik, Andrzej$$b1
000842649 7001_ $$0P:(DE-HGF)0$$aEble, Michael$$b2
000842649 7001_ $$0P:(DE-HGF)0$$aPinkawa, Michael$$b3
000842649 7001_ $$0P:(DE-Juel1)133469$$aKriehuber, Ralf$$b4$$ufzj
000842649 7001_ $$0P:(DE-Juel1)133346$$aSchmitz, Sabine$$b5$$ufzj
000842649 8564_ $$uhttps://juser.fz-juelich.de/record/842649/files/Brzozowska%20et%20al%20ERRS%202009%20Prague.pdf$$yOpenAccess
000842649 8564_ $$uhttps://juser.fz-juelich.de/record/842649/files/Brzozowska%20et%20al%20ERRS%202009%20Prague.gif?subformat=icon$$xicon$$yOpenAccess
000842649 8564_ $$uhttps://juser.fz-juelich.de/record/842649/files/Brzozowska%20et%20al%20ERRS%202009%20Prague.jpg?subformat=icon-1440$$xicon-1440$$yOpenAccess
000842649 8564_ $$uhttps://juser.fz-juelich.de/record/842649/files/Brzozowska%20et%20al%20ERRS%202009%20Prague.jpg?subformat=icon-180$$xicon-180$$yOpenAccess
000842649 8564_ $$uhttps://juser.fz-juelich.de/record/842649/files/Brzozowska%20et%20al%20ERRS%202009%20Prague.jpg?subformat=icon-640$$xicon-640$$yOpenAccess
000842649 8564_ $$uhttps://juser.fz-juelich.de/record/842649/files/Brzozowska%20et%20al%20ERRS%202009%20Prague.pdf?subformat=pdfa$$xpdfa$$yOpenAccess
000842649 909CO $$ooai:juser.fz-juelich.de:842649$$pdriver$$pVDB$$popen_access$$popenaire
000842649 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-HGF)0$$aForschungszentrum Jülich$$b0$$kFZJ
000842649 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a S-US$$b0
000842649 9101_ $$0I:(DE-588b)36225-6$$6P:(DE-HGF)0$$aRWTH Aachen$$b2$$kRWTH
000842649 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Universitätsklinik für Strahlentherapie$$b2
000842649 9101_ $$0I:(DE-588b)36225-6$$6P:(DE-HGF)0$$aRWTH Aachen$$b3$$kRWTH
000842649 9101_ $$0I:(DE-HGF)0$$6P:(DE-HGF)0$$a Universitätsklinik für Strahlentherapie$$b3
000842649 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133469$$aForschungszentrum Jülich$$b4$$kFZJ
000842649 9101_ $$0I:(DE-588b)5008462-8$$6P:(DE-Juel1)133346$$aForschungszentrum Jülich$$b5$$kFZJ
000842649 9131_ $$0G:(DE-HGF)POF3-899$$1G:(DE-HGF)POF3-890$$2G:(DE-HGF)POF3-800$$3G:(DE-HGF)POF3$$4G:(DE-HGF)POF$$aDE-HGF$$bProgrammungebundene Forschung$$lohne Programm$$vohne Topic$$x0
000842649 915__ $$0StatID:(DE-HGF)0510$$2StatID$$aOpenAccess
000842649 920__ $$lyes
000842649 9201_ $$0I:(DE-Juel1)S-US-20090406$$kS-US$$lSicherheit und Strahlenschutz, Umgebungsüberwachung,Strahlenbiologie$$x0
000842649 980__ $$aposter
000842649 980__ $$aVDB
000842649 980__ $$aUNRESTRICTED
000842649 980__ $$aI:(DE-Juel1)S-US-20090406
000842649 9801_ $$aFullTexts