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@INPROCEEDINGS{Brzozowska:842649,
      author       = {Brzozowska, Kinga and Wojcik, Andrzej and Eble, Michael and
                      Pinkawa, Michael and Kriehuber, Ralf and Schmitz, Sabine},
      title        = {{C}omparison of individual radiosensitivity of peripheral
                      blood lymphocytes from prostate cancer patients and healthy
                      donors},
      reportid     = {FZJ-2018-00856},
      year         = {2009},
      abstract     = {Introduction: There is some evidence that approximately
                      $10\%$ of the population show an enhanced intrinsic
                      radiosensitivity of normal tissue and hence have a higher
                      risk for developing side-effects during or after
                      radiotherapy. For that reason there is a need for a fast and
                      robust test to assess normal tissue response to
                      radiotherapy. DNA repair deficiency and altered apoptosis
                      characteristics are discussed as promising markers of
                      radiosensitivity. The aim of our study was to find out
                      whether peripheral blood lymphocytes (PBLs) from cancer
                      patients with strong clinical side effects following
                      radiotherapy as assessed clinically on the basis of the
                      RTOG/EORTC scale, show enhanced rates of in vitro
                      radiation-induced double strand breaks (dsb), decreased DNA
                      repair capacity and altered induction of apoptosis when
                      compared to lymphocytes from patients without side effects
                      and healthy age–matched donors. Materials and methods: The
                      study included 20 prostate cancer patients without and 20
                      patients with acute side-effects during and after
                      radiothrapy, as well as 20 healthy age-matched donors. From
                      each donor, blood samples were collected, exposed to a
                      radiation dose of 0.5 Gy or 1 Gy γ –rays and analysed for
                      the following biological endpoints: the initial level of dsb
                      and the repair kinetics (γ–H2AX–assay), apoptosis
                      (Annexin V/PI–assay) and the induction of chromatid–type
                      chromosomal aberrations (G2–assay). Additionally FISH
                      analysis (G0-aberrations) were performed for some patients
                      of each group. Results: The maximum number of γ–H2AX foci
                      occurred at 30 min after exposure without significant
                      differences in numbers of foci between both groups of
                      patients and healthy donors. The frequency of spontaneous
                      and radiation-induced apoptoses was determined 30 min, 5 h
                      and 24 h post- irradiation and showed a significant increase
                      of early apoptosis 24 h post-irradiation for both patients
                      and healthy donors. The rate of necrosis had a tendency to
                      increase 24 h post-irradiation in patients with strong
                      clinical side effects. The highest level of aberrations was
                      observed for patients with strong clinical side effects in
                      comparison to patients without any side effects and healthy
                      donors.},
      month         = {Aug},
      date          = {2009-08-26},
      organization  = {37th Annual Meeting of the European
                       Radiation Research Society, Prague
                       (Czech Republic), 26 Aug 2009 - 29 Aug
                       2009},
      subtyp        = {After Call},
      cin          = {S-US},
      cid          = {I:(DE-Juel1)S-US-20090406},
      pnm          = {899 - ohne Topic (POF3-899)},
      pid          = {G:(DE-HGF)POF3-899},
      typ          = {PUB:(DE-HGF)24},
      url          = {https://juser.fz-juelich.de/record/842649},
}