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@ARTICLE{Katranidis:842811,
      author       = {Katranidis, Alexandros and Sadoine, Mayuri and Cerminara,
                      M. and Gerrits, M. and Fitter, Jörg},
      title        = {{C}o-translational incorporation into proteins of a
                      fluorophore suitable for sm{FRET} studies},
      journal      = {ACS synthetic biology},
      volume       = {7},
      number       = {2},
      issn         = {2161-5063},
      address      = {Washington, DC},
      publisher    = {ACS},
      reportid     = {FZJ-2018-01007},
      pages        = {405–411},
      year         = {2018},
      abstract     = {Single-molecule FRET (smFRET) is a powerful tool to
                      investigate conformational changes of biological molecules.
                      In general, smFRET studies require protein samples that are
                      site-specifically double-labeled with a pair of donor and
                      acceptor fluorophores. The common approaches to produce such
                      samples cannot be applied when studying the synthesis and
                      folding of the polypeptide chain on the ribosome. The best
                      strategy is to incorporate two fluorescent amino acids
                      cotranslationally using cell-free protein synthesis systems.
                      Here, we demonstrate the cotranslational site-specific
                      incorporation into a model protein of Atto633, a dye with
                      excellent photophysical properties, suitable for single
                      molecule spectroscopy, together with a second dye using a
                      combination of the sense cysteine and the nonsense amber
                      codon. In this work we show that cotranslational
                      incorporation of good fluorophores into proteins is a viable
                      strategy to produce suitable samples for smFRET studies.},
      cin          = {ICS-5},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-5-20110106},
      pnm          = {551 - Functional Macromolecules and Complexes (POF3-551)},
      pid          = {G:(DE-HGF)POF3-551},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29370697},
      UT           = {WOS:000426012600013},
      doi          = {10.1021/acssynbio.7b00433},
      url          = {https://juser.fz-juelich.de/record/842811},
}