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@ARTICLE{Karmi:843804,
author = {Karmi, Ola and Marjault, Henri-Baptiste and Pesce, Luca and
Carloni, Paolo and Onuchic, Jose’ N. and Jennings,
Patricia A. and Mittler, Ron and Nechushtai, Rachel},
title = {{T}he unique fold and lability of the [2{F}e-2{S}] clusters
of {NEET} proteins mediate their key functions in health and
disease},
journal = {Journal of biological inorganic chemistry},
volume = {23},
number = {4},
issn = {1432-1327},
address = {Berlin},
publisher = {Springer},
reportid = {FZJ-2018-01344},
pages = {599-612},
year = {2018},
abstract = {NEET proteins comprise a new class of [2Fe-2S] cluster
proteins. In human, three genes encode for NEET proteins:
cisd1 encodes mitoNEET (mNT), cisd2 encodes the
Nutrient-deprivation autophagy factor-1 (NAF-1) and cisd3
encodes MiNT (Miner2). These recently discovered proteins
play key roles in many processes related to normal
metabolism and disease. Indeed, NEET proteins are involved
in iron, Fe-S, and reactive oxygen homeostasis in cells and
play an important role in regulating apoptosis and
autophagy. mNT and NAF-1 are homodimeric and reside on the
outer mitochondrial membrane. NAF-1 also resides in the
membranes of the ER associated mitochondrial membranes (MAM)
and the ER. MiNT is a monomer with distinct asymmetry in the
molecular surfaces surrounding the clusters. Unlike its
paralogs mNT and NAF-1, it resides within the mitochondria.
NAF-1 and mNT share similar backbone folds to the plant
homodimeric NEET protein (At-NEET), while MiNT’s backbone
fold resembles a bacterial MiNT protein. Despite the
variation of amino acid composition among these proteins,
all NEET proteins retained their unique CDGSH domain
harboring their unique 3Cys:1His [2Fe-2S] cluster
coordination through evolution. The coordinating exposed His
was shown to convey the lability to the NEET proteins’
[2Fe-2S] clusters. In this minireview, we discuss the NEET
fold and its structural elements. Special attention is given
to the unique lability of the NEETs’ [2Fe-2S] cluster and
the implication of the latter to the NEET proteins’
cellular and systemic function in health and disease},
cin = {IAS-5 / INM-9},
ddc = {540},
cid = {I:(DE-Juel1)IAS-5-20120330 / I:(DE-Juel1)INM-9-20140121},
pnm = {571 - Connectivity and Activity (POF3-571)},
pid = {G:(DE-HGF)POF3-571},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29435647},
UT = {WOS:000435600800012},
doi = {10.1007/s00775-018-1538-8},
url = {https://juser.fz-juelich.de/record/843804},
}