TY  - JOUR
AU  - Hornung, Raphael
AU  - Grünberger, Alexander
AU  - Westerwalbesloh, Christoph
AU  - Kohlheyer, Dietrich
AU  - Gompper, Gerhard
AU  - Elgeti, Jens
TI  - Quantitative modelling of nutrient-limited growth of bacterial colonies in microfluidic cultivation
JO  - Interface
VL  - 15
IS  - 139
SN  - 1742-5662
CY  - London
PB  - The Royal Society
M1  - FZJ-2018-01366
SP  - 20170713 -
PY  - 2018
AB  - Nutrient gradients and limitations play a pivotal role in the life of all microbes, both in their natural habitat as well as in artificial, microfluidic systems. Spatial concentration gradients of nutrients in densely packed cell configurations may locally affect the bacterial growth leading to heterogeneous micropopulations. A detailed understanding and quantitative modelling of cellular behaviour under nutrient limitations is thus highly desirable. We use microfluidic cultivations to investigate growth and microbial behaviour of the model organism Corynebacterium glutamicum under well-controlled conditions. With a reaction–diffusion-type model, parameters are extracted from steady-state experiments with a one-dimensional nutrient gradient. Subsequently, we employ particle-based simulations with these parameters to predict the dynamical growth of a colony in two dimensions. Comparing the results of those simulations with microfluidic experiments yields excellent agreement. Our modelling approach lays the foundation for a better understanding of dynamic microbial growth processes, both in nature and in applied biotechnology.
LB  - PUB:(DE-HGF)16
UR  - <Go to ISI:>//WOS:000426464000003
C6  - pmid:29445038
DO  - DOI:10.1098/rsif.2017.0713
UR  - https://juser.fz-juelich.de/record/843827
ER  -