TY  - JOUR
AU  - Maslov, Ivan
AU  - Bogorodskiy, Andrey
AU  - Mishin, Alexey
AU  - Okhrimenko, Ivan
AU  - Gushchin, Ivan
AU  - Kalenov, Sergei
AU  - Dencher, Norbert A.
AU  - Fahlke, Christoph
AU  - Büldt, Georg
AU  - Gordeliy, Valentin
AU  - Gensch, Thomas
AU  - Borshchevskiy, Valentin
TI  - Efficient non-cytotoxic fluorescent staining of halophiles
JO  - Scientific reports
VL  - 8
IS  - 1
SN  - 2045-2322
CY  - London
PB  - Nature Publishing Group
M1  - FZJ-2018-01392
SP  - 2549
PY  - 2018
AB  - Research on halophilic microorganisms is important due to their relation to fundamental questions of survival of living organisms in a hostile environment. Here we introduce a novel method to stain halophiles with MitoTracker fluorescent dyes in their growth medium. The method is based on membrane-potential sensitive dyes, which were originally used to label mitochondria in eukaryotic cells. We demonstrate that these fluorescent dyes provide high staining efficiency and are beneficial for multi-staining purposes due to the spectral range covered (from orange to deep red). In contrast with other fluorescent dyes used so far, MitoTracker does not affect growth rate, and remains in cells after several washing steps and several generations in cell culture. The suggested dyes were tested on three archaeal (Hbt. salinarum, Haloferax sp., Halorubrum sp.) and two bacterial (Salicola sp., Halomonas sp.) strains of halophilic microorganisms. The new staining approach provides new insights into biology of Hbt. salinarum. We demonstrated the interconversion of rod-shaped cells of Hbt. salinarium to spheroplasts and submicron-sized spheres, as well as the cytoplasmic integrity of giant rod Hbt. salinarum species. By expanding the variety of tools available for halophile detection, MitoTracker dyes overcome long-standing limitations in fluorescence microscopy studies of halophiles.
LB  - PUB:(DE-HGF)16
C6  - pmid:29416075
UR  - <Go to ISI:>//WOS:000424318700021
DO  - DOI:10.1038/s41598-018-20839-7
UR  - https://juser.fz-juelich.de/record/843861
ER  -