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@ARTICLE{Maslov:843861,
      author       = {Maslov, Ivan and Bogorodskiy, Andrey and Mishin, Alexey and
                      Okhrimenko, Ivan and Gushchin, Ivan and Kalenov, Sergei and
                      Dencher, Norbert A. and Fahlke, Christoph and Büldt, Georg
                      and Gordeliy, Valentin and Gensch, Thomas and Borshchevskiy,
                      Valentin},
      title        = {{E}fficient non-cytotoxic fluorescent staining of
                      halophiles},
      journal      = {Scientific reports},
      volume       = {8},
      number       = {1},
      issn         = {2045-2322},
      address      = {London},
      publisher    = {Nature Publishing Group},
      reportid     = {FZJ-2018-01392},
      pages        = {2549},
      year         = {2018},
      abstract     = {Research on halophilic microorganisms is important due to
                      their relation to fundamental questions of survival of
                      living organisms in a hostile environment. Here we introduce
                      a novel method to stain halophiles with MitoTracker
                      fluorescent dyes in their growth medium. The method is based
                      on membrane-potential sensitive dyes, which were originally
                      used to label mitochondria in eukaryotic cells. We
                      demonstrate that these fluorescent dyes provide high
                      staining efficiency and are beneficial for multi-staining
                      purposes due to the spectral range covered (from orange to
                      deep red). In contrast with other fluorescent dyes used so
                      far, MitoTracker does not affect growth rate, and remains in
                      cells after several washing steps and several generations in
                      cell culture. The suggested dyes were tested on three
                      archaeal (Hbt. salinarum, Haloferax sp., Halorubrum sp.) and
                      two bacterial (Salicola sp., Halomonas sp.) strains of
                      halophilic microorganisms. The new staining approach
                      provides new insights into biology of Hbt. salinarum. We
                      demonstrated the interconversion of rod-shaped cells of Hbt.
                      salinarium to spheroplasts and submicron-sized spheres, as
                      well as the cytoplasmic integrity of giant rod Hbt.
                      salinarum species. By expanding the variety of tools
                      available for halophile detection, MitoTracker dyes overcome
                      long-standing limitations in fluorescence microscopy studies
                      of halophiles.},
      cin          = {ICS-4 / ICS-6},
      ddc          = {000},
      cid          = {I:(DE-Juel1)ICS-4-20110106 / I:(DE-Juel1)ICS-6-20110106},
      pnm          = {553 - Physical Basis of Diseases (POF3-553)},
      pid          = {G:(DE-HGF)POF3-553},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29416075},
      UT           = {WOS:000424318700021},
      doi          = {10.1038/s41598-018-20839-7},
      url          = {https://juser.fz-juelich.de/record/843861},
}