Home > Publications database > Unravelling the Di- and Oligomerisation Interfaces of the G-Protein Coupled Bile Acid Receptor TGR5 via Integrative Modelling > print

001     844071
005     20210129232808.0
024 7 _ |a 2128/17547
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037 _ _ |a FZJ-2018-01580
100 1 _ |a Gertzen, C. G. W.
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111 2 _ |a NIC Symposium 2018
|c Jülich
|d 2018-02-22 - 2018-02-23
|w Germany
245 _ _ |a Unravelling the Di- and Oligomerisation Interfaces of the G-Protein Coupled Bile Acid Receptor TGR5 via Integrative Modelling
260 _ _ |a Jülich
|c 2018
|b John von Neumann Institute for Computing
300 _ _ |a 25 - 31
336 7 _ |a CONFERENCE_PAPER
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490 0 _ |a Publication Series of the John von Neumann Institute for Computing (NIC) NIC Series
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520 _ _ |a TGR5 is a bile acid- and neurosteroid-sensing G-protein coupled receptor (GPCR), which isalmost ubiquitously expressed throughout the human body. Its physiological functions comprisethe regulation of blood glucose homeostasis, metabolism, and inflammation. Additionally,recent studies show an involvement of TGR5 in the formation of gastric, esophageal, andcholangiocyte cancers as well as in bile acid-induced itch. Hence, TGR5 has been identified asan important drug target. To reduce side effects of drugs targeting GPCRs, the development ofbivalent ligands specifically targeting dimers was shown to be promising. To do so, the knowledgeof the dimerisation interfaces of these GPCRs is paramount. However, the dimerisationinterfaces of TGR5 are not known. Here, we present the identification of the primary dimerisationinterface of TGR5 and possible oligomerisation interfaces. We used Multiparameter ImageFluorescence Spectroscopy (MFIS) Förster Resonance Energy Transfer (FRET) measurementsof fluorescently labelled TGR5 in live cells to measure apparent distances between two TGR5protomers and compared them to distances computed for putative TGR5 dimer models. Asthe linker between TGR5 and the fluorophores contained more than 30 residues, we used all-atommolecular dynamics (MD) simulations to sample the conformational space of the linkerand fluorophore in relation to TGR5. The sampled configurations were reweighted by free energycalculations using the molecular mechanics Poisson-Boltzmann surface area (MM-PBSA)method to account for the presence of solvent and a membrane, and a random energy model toestimate the configurational entropy. This allowed us to identify the 1-8 interface of TGR5 asthe primary dimerisation interface, with the 4-5 and 5-6 interfaces as possible oligomerisationsites. This information might be used to develop novel TGR5 ligands with a reduced side-effectprofile.
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536 _ _ |a Antagonists of the TGR5 G-protein complex formation (hdd15_20170501)
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536 _ _ |a Energetics of the dimerization and G-protein coupling of the bile-acid sensing GPCR TGR5 (hdd15_20160501)
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|f Energetics of the dimerization and G-protein coupling of the bile-acid sensing GPCR TGR5
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700 1 _ |a Keitel, V.
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700 1 _ |a Seidel, C. A. M.
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700 1 _ |a Gohlke, H.
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