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@ARTICLE{Kulawik:844306,
author = {Kulawik, Andreas and Heise, Henrike and Zafiu, Christian
and Willbold, Dieter and Bannach, Oliver},
title = {{A}dvancements of the s{FIDA} method for oligomer-based
diagnostics of neurodegenerative diseases},
journal = {FEBS letters},
volume = {592},
number = {4},
issn = {0014-5793},
address = {Chichester},
publisher = {Wiley},
reportid = {FZJ-2018-01739},
pages = {516 - 534},
year = {2018},
abstract = {Early diagnosis of Alzheimer's disease (AD) is of great
importance for the development of therapeutics and their
application in the clinical environment. Amyloid β (Aβ)
oligomers are crucial for the onset and progression of AD
and represent a popular drug target, being presumably the
most direct biomarker. Efforts to measure Aβ oligomers in
body fluids are hampered by the low analyte concentration
and presence of Aβ monomers. The surface-based fluorescence
intensity distribution analysis (sFIDA) features both highly
specific and sensitive oligomer quantitation as well as
total insensitivity towards monomers. In this Review, we
highlight structural features of oligomeric and fibrillar
Aβ. Recent advancements in sFIDA assay development have
been the successful automation, adaption for additional
biomarkers such as α-synuclein oligomers, and significant
improvement of essential assay parameters.},
cin = {ICS-6},
ddc = {570},
cid = {I:(DE-Juel1)ICS-6-20110106},
pnm = {553 - Physical Basis of Diseases (POF3-553)},
pid = {G:(DE-HGF)POF3-553},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29360176},
UT = {WOS:000426183900006},
doi = {10.1002/1873-3468.12983},
url = {https://juser.fz-juelich.de/record/844306},
}