TY  - JOUR
AU  - Kugler, Eva Maria
AU  - Michel, Klaus
AU  - Kirchenbüchler, David
AU  - Dreissen, Georg
AU  - Csiszár, Agnes
AU  - Merkel, Rudolf
AU  - Schemann, Michael
AU  - Mazzuoli-Weber, Gemma
TI  - Sensitivity to Strain and Shear Stress of Isolated Mechanosensitive Enteric Neurons
JO  - Neuroscience
VL  - 372
SN  - 0306-4522
CY  - Amsterdam [u.a.]
PB  - Elsevier Science
M1  - FZJ-2018-02230
SP  - 213 - 224
PY  - 2018
AB  - Within the enteric nervous system, the neurons in charge to control motility of the gastrointestinal tract reside in a particular location nestled between two perpendicular muscle layers which contract and relax. We used primary cultured myenteric neurons of male guinea pigs to study mechanosensitivity of enteric neurons in isolation. Ultrafast Neuroimaging with a voltage-sensitive dye technique was used to record neuronal activity in response to shear stress and strain. Strain was induced by locally deforming the elastic cell culture substrate next to a neuron. Measurements showed that substrate strain was mostly elongating cells. Shear stress was exerted by hydrodynamic forces in a microchannel. Both stimuli induced excitatory responses. Strain activated 14% of the stimulated myenteric neurons that responded with a spike frequency of 1.9 (0.7/3.2) Hz, whereas shear stress excited only a few neurons (5.6%) with a very low spike frequency of 0 (0/0.6) Hz. Thus, shear stress does not seem to be an adequate stimulus for mechanosensitive enteric neurons (MEN) while strain activates enteric neurons in a relevant manner. Analyzing the adaptation behavior of MEN showed that shear stress activated rapidly/slowly/ultraslowly adapting MEN (2/62/36%) whereas strain only slowly (46%) and ultraslowly (54%) MEN. Paired experiments with strain and normal stress revealed three mechanosensitive enteric neuronal populations: one strain-sensitive (37%), one normal stress-sensitive (17%) and one strain- and stress-sensitive (46%).These results indicate that shear stress does not play a role in the neuronal control of motility but normal stress and strain.
LB  - PUB:(DE-HGF)16
C6  - pmid:29317262
UR  - <Go to ISI:>//WOS:000425878100017
DO  - DOI:10.1016/j.neuroscience.2017.12.052
UR  - https://juser.fz-juelich.de/record/844884
ER  -