% IMPORTANT: The following is UTF-8 encoded.  This means that in the presence
% of non-ASCII characters, it will not work with BibTeX 0.99 or older.
% Instead, you should use an up-to-date BibTeX implementation like “bibtex8” or
% “biber”.

@ARTICLE{Kugler:844884,
      author       = {Kugler, Eva Maria and Michel, Klaus and Kirchenbüchler,
                      David and Dreissen, Georg and Csiszár, Agnes and Merkel,
                      Rudolf and Schemann, Michael and Mazzuoli-Weber, Gemma},
      title        = {{S}ensitivity to {S}train and {S}hear {S}tress of
                      {I}solated {M}echanosensitive {E}nteric {N}eurons},
      journal      = {Neuroscience},
      volume       = {372},
      issn         = {0306-4522},
      address      = {Amsterdam [u.a.]},
      publisher    = {Elsevier Science},
      reportid     = {FZJ-2018-02230},
      pages        = {213 - 224},
      year         = {2018},
      abstract     = {Within the enteric nervous system, the neurons in charge to
                      control motility of the gastrointestinal tract reside in a
                      particular location nestled between two perpendicular muscle
                      layers which contract and relax. We used primary cultured
                      myenteric neurons of male guinea pigs to study
                      mechanosensitivity of enteric neurons in isolation.
                      Ultrafast Neuroimaging with a voltage-sensitive dye
                      technique was used to record neuronal activity in response
                      to shear stress and strain. Strain was induced by locally
                      deforming the elastic cell culture substrate next to a
                      neuron. Measurements showed that substrate strain was mostly
                      elongating cells. Shear stress was exerted by hydrodynamic
                      forces in a microchannel. Both stimuli induced excitatory
                      responses. Strain activated $14\%$ of the stimulated
                      myenteric neurons that responded with a spike frequency of
                      1.9 (0.7/3.2) Hz, whereas shear stress excited only a few
                      neurons $(5.6\%)$ with a very low spike frequency of 0
                      (0/0.6) Hz. Thus, shear stress does not seem to be an
                      adequate stimulus for mechanosensitive enteric neurons (MEN)
                      while strain activates enteric neurons in a relevant manner.
                      Analyzing the adaptation behavior of MEN showed that shear
                      stress activated rapidly/slowly/ultraslowly adapting MEN
                      $(2/62/36\%)$ whereas strain only slowly $(46\%)$ and
                      ultraslowly $(54\%)$ MEN. Paired experiments with strain and
                      normal stress revealed three mechanosensitive enteric
                      neuronal populations: one strain-sensitive $(37\%),$ one
                      normal stress-sensitive $(17\%)$ and one strain- and
                      stress-sensitive $(46\%).These$ results indicate that shear
                      stress does not play a role in the neuronal control of
                      motility but normal stress and strain.},
      cin          = {ICS-7},
      ddc          = {610},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29317262},
      UT           = {WOS:000425878100017},
      doi          = {10.1016/j.neuroscience.2017.12.052},
      url          = {https://juser.fz-juelich.de/record/844884},
}