guest ::
| Home > Publications database > Sensitivity to Strain and Shear Stress of Isolated Mechanosensitive Enteric Neurons > print |
| Home > Publications database > Sensitivity to Strain and Shear Stress of Isolated Mechanosensitive Enteric Neurons > print |
| 001 | 844884 | ||
| 005 | 20210129233131.0 | ||
| 024 | 7 | _ | |a 10.1016/j.neuroscience.2017.12.052 |2 doi |
| 024 | 7 | _ | |a 0306-4522 |2 ISSN |
| 024 | 7 | _ | |a 1873-7544 |2 ISSN |
| 024 | 7 | _ | |a pmid:29317262 |2 pmid |
| 024 | 7 | _ | |a WOS:000425878100017 |2 WOS |
| 037 | _ | _ | |a FZJ-2018-02230 |
| 041 | _ | _ | |a English |
| 082 | _ | _ | |a 610 |
| 100 | 1 | _ | |a Kugler, Eva Maria |0 P:(DE-HGF)0 |b 0 |
| 245 | _ | _ | |a Sensitivity to Strain and Shear Stress of Isolated Mechanosensitive Enteric Neurons |
| 260 | _ | _ | |a Amsterdam [u.a.] |c 2018 |b Elsevier Science |
| 336 | 7 | _ | |a article |2 DRIVER |
| 336 | 7 | _ | |a Output Types/Journal article |2 DataCite |
| 336 | 7 | _ | |a Journal Article |b journal |m journal |0 PUB:(DE-HGF)16 |s 1523208235_27708 |2 PUB:(DE-HGF) |
| 336 | 7 | _ | |a ARTICLE |2 BibTeX |
| 336 | 7 | _ | |a JOURNAL_ARTICLE |2 ORCID |
| 336 | 7 | _ | |a Journal Article |0 0 |2 EndNote |
| 520 | _ | _ | |a Within the enteric nervous system, the neurons in charge to control motility of the gastrointestinal tract reside in a particular location nestled between two perpendicular muscle layers which contract and relax. We used primary cultured myenteric neurons of male guinea pigs to study mechanosensitivity of enteric neurons in isolation. Ultrafast Neuroimaging with a voltage-sensitive dye technique was used to record neuronal activity in response to shear stress and strain. Strain was induced by locally deforming the elastic cell culture substrate next to a neuron. Measurements showed that substrate strain was mostly elongating cells. Shear stress was exerted by hydrodynamic forces in a microchannel. Both stimuli induced excitatory responses. Strain activated 14% of the stimulated myenteric neurons that responded with a spike frequency of 1.9 (0.7/3.2) Hz, whereas shear stress excited only a few neurons (5.6%) with a very low spike frequency of 0 (0/0.6) Hz. Thus, shear stress does not seem to be an adequate stimulus for mechanosensitive enteric neurons (MEN) while strain activates enteric neurons in a relevant manner. Analyzing the adaptation behavior of MEN showed that shear stress activated rapidly/slowly/ultraslowly adapting MEN (2/62/36%) whereas strain only slowly (46%) and ultraslowly (54%) MEN. Paired experiments with strain and normal stress revealed three mechanosensitive enteric neuronal populations: one strain-sensitive (37%), one normal stress-sensitive (17%) and one strain- and stress-sensitive (46%).These results indicate that shear stress does not play a role in the neuronal control of motility but normal stress and strain. |
| 536 | _ | _ | |a 552 - Engineering Cell Function (POF3-552) |0 G:(DE-HGF)POF3-552 |c POF3-552 |f POF III |x 0 |
| 588 | _ | _ | |a Dataset connected to CrossRef |
| 700 | 1 | _ | |a Michel, Klaus |0 P:(DE-HGF)0 |b 1 |
| 700 | 1 | _ | |a Kirchenbüchler, David |0 P:(DE-HGF)0 |b 2 |
| 700 | 1 | _ | |a Dreissen, Georg |0 P:(DE-Juel1)129308 |b 3 |
| 700 | 1 | _ | |a Csiszár, Agnes |0 P:(DE-Juel1)128805 |b 4 |
| 700 | 1 | _ | |a Merkel, Rudolf |0 P:(DE-Juel1)128833 |b 5 |
| 700 | 1 | _ | |a Schemann, Michael |0 P:(DE-HGF)0 |b 6 |
| 700 | 1 | _ | |a Mazzuoli-Weber, Gemma |0 P:(DE-HGF)0 |b 7 |e Corresponding author |
| 773 | _ | _ | |a 10.1016/j.neuroscience.2017.12.052 |g Vol. 372, p. 213 - 224 |0 PERI:(DE-600)1498423-4 |p 213 - 224 |t Neuroscience |v 372 |y 2018 |x 0306-4522 |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/844884/files/1-s2.0-S0306452218300022-main.pdf |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/844884/files/1-s2.0-S0306452218300022-main.gif?subformat=icon |x icon |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/844884/files/1-s2.0-S0306452218300022-main.jpg?subformat=icon-1440 |x icon-1440 |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/844884/files/1-s2.0-S0306452218300022-main.jpg?subformat=icon-180 |x icon-180 |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/844884/files/1-s2.0-S0306452218300022-main.jpg?subformat=icon-640 |x icon-640 |y Restricted |
| 856 | 4 | _ | |u https://juser.fz-juelich.de/record/844884/files/1-s2.0-S0306452218300022-main.pdf?subformat=pdfa |x pdfa |y Restricted |
| 909 | C | O | |o oai:juser.fz-juelich.de:844884 |p VDB |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 2 |6 P:(DE-HGF)0 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 3 |6 P:(DE-Juel1)129308 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 4 |6 P:(DE-Juel1)128805 |
| 910 | 1 | _ | |a Forschungszentrum Jülich |0 I:(DE-588b)5008462-8 |k FZJ |b 5 |6 P:(DE-Juel1)128833 |
| 913 | 1 | _ | |a DE-HGF |b Key Technologies |l BioSoft – Fundamentals for future Technologies in the fields of Soft Matter and Life Sciences |1 G:(DE-HGF)POF3-550 |0 G:(DE-HGF)POF3-552 |2 G:(DE-HGF)POF3-500 |v Engineering Cell Function |x 0 |4 G:(DE-HGF)POF |3 G:(DE-HGF)POF3 |
| 914 | 1 | _ | |y 2018 |
| 915 | _ | _ | |a Nationallizenz |0 StatID:(DE-HGF)0420 |2 StatID |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0300 |2 StatID |b Medline |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0310 |2 StatID |b NCBI Molecular Biology Database |
| 915 | _ | _ | |a JCR |0 StatID:(DE-HGF)0100 |2 StatID |b NEUROSCIENCE : 2015 |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0200 |2 StatID |b SCOPUS |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0600 |2 StatID |b Ebsco Academic Search |
| 915 | _ | _ | |a Peer Review |0 StatID:(DE-HGF)0030 |2 StatID |b ASC |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0199 |2 StatID |b Thomson Reuters Master Journal List |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0110 |2 StatID |b Science Citation Index |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)0150 |2 StatID |b Web of Science Core Collection |
| 915 | _ | _ | |a WoS |0 StatID:(DE-HGF)0111 |2 StatID |b Science Citation Index Expanded |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1030 |2 StatID |b Current Contents - Life Sciences |
| 915 | _ | _ | |a DBCoverage |0 StatID:(DE-HGF)1050 |2 StatID |b BIOSIS Previews |
| 915 | _ | _ | |a IF < 5 |0 StatID:(DE-HGF)9900 |2 StatID |
| 920 | _ | _ | |l yes |
| 920 | 1 | _ | |0 I:(DE-Juel1)ICS-7-20110106 |k ICS-7 |l Biomechanik |x 0 |
| 980 | _ | _ | |a journal |
| 980 | _ | _ | |a VDB |
| 980 | _ | _ | |a I:(DE-Juel1)ICS-7-20110106 |
| 980 | _ | _ | |a UNRESTRICTED |
| 981 | _ | _ | |a I:(DE-Juel1)IBI-2-20200312 |
| Library | Collection | CLSMajor | CLSMinor | Language | Author |
|---|