TY  - JOUR
AU  - Sreij, Ramsia
AU  - Dargel, Carina
AU  - Geisler, Philippe
AU  - Hertle, Yvonne
AU  - Radulescu, Aurel
AU  - Pasini, Stefano
AU  - Perez, Javier
AU  - Moleiro, Lara H.
AU  - Hellweg, Thomas
TI  - DMPC vesicle structure and dynamics in the presence of low amounts of the saponin aescin
JO  - Physical chemistry, chemical physics
VL  - 20
IS  - 14
SN  - 1463-9084
CY  - Cambridge
PB  - RSC Publ.
M1  - FZJ-2018-02271
SP  - 9070 - 9083
PY  - 2018
AB  - Vesicle shape and bilayer parameters are studied by small-angle X-ray (SAXS) and small-angle neutron (SANS) scattering in the presence of the saponin aescin. We confirm successful incorporation of aescin molecules by analysis of the radii of gyration RG and study furthermore the impact of aescin incorporation on bilayer thickness parameters from the neutron and X-ray perspective. Additionally, the bending elasticity (κ) of these 1,2-dimyristoyl-sn-glycero-3-phosphocholine vesicle bilayers is studied in the presence of aescin. Neutron spin-echo spectroscopy (NSE) allows to detect subtle changes in the dynamics and κ of lipid membranes. Changes of κ are detectable at temperatures below and above the main phase transition temperature Tm of the lipid. The impact of aescin is much more significant below Tm. It has been found that below Tm the addition of aescin to the vesicles decreases the value of κ and softens the bilayer. Above Tm the value of κ increases with increasing aescin content and the bilayer becomes more rigid. Altogether, we demonstrate by analysis of the structure and dynamics of the vesicles that the impact of aescin strongly depends on the lipid state. Below Tm the membrane becomes fluidized and softer, above Tm solidified and stiffer compared to a DMPC membrane without additive at similar conditions.
LB  - PUB:(DE-HGF)16
C6  - pmid:29505043
UR  - <Go to ISI:>//WOS:000429205700005
DO  - DOI:10.1039/C7CP08027A
UR  - https://juser.fz-juelich.de/record/844929
ER  -