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@ARTICLE{Sreij:844929,
author = {Sreij, Ramsia and Dargel, Carina and Geisler, Philippe and
Hertle, Yvonne and Radulescu, Aurel and Pasini, Stefano and
Perez, Javier and Moleiro, Lara H. and Hellweg, Thomas},
title = {{DMPC} vesicle structure and dynamics in the presence of
low amounts of the saponin aescin},
journal = {Physical chemistry, chemical physics},
volume = {20},
number = {14},
issn = {1463-9084},
address = {Cambridge},
publisher = {RSC Publ.},
reportid = {FZJ-2018-02271},
pages = {9070 - 9083},
year = {2018},
abstract = {Vesicle shape and bilayer parameters are studied by
small-angle X-ray (SAXS) and small-angle neutron (SANS)
scattering in the presence of the saponin aescin. We confirm
successful incorporation of aescin molecules by analysis of
the radii of gyration RG and study furthermore the impact of
aescin incorporation on bilayer thickness parameters from
the neutron and X-ray perspective. Additionally, the bending
elasticity (κ) of these
1,2-dimyristoyl-sn-glycero-3-phosphocholine vesicle bilayers
is studied in the presence of aescin. Neutron spin-echo
spectroscopy (NSE) allows to detect subtle changes in the
dynamics and κ of lipid membranes. Changes of κ are
detectable at temperatures below and above the main phase
transition temperature Tm of the lipid. The impact of aescin
is much more significant below Tm. It has been found that
below Tm the addition of aescin to the vesicles decreases
the value of κ and softens the bilayer. Above Tm the value
of κ increases with increasing aescin content and the
bilayer becomes more rigid. Altogether, we demonstrate by
analysis of the structure and dynamics of the vesicles that
the impact of aescin strongly depends on the lipid state.
Below Tm the membrane becomes fluidized and softer, above Tm
solidified and stiffer compared to a DMPC membrane without
additive at similar conditions.},
cin = {JCNS (München) ; Jülich Centre for Neutron Science JCNS
(München) ; JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
ddc = {540},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
experiment = {EXP:(DE-MLZ)KWS2-20140101 / EXP:(DE-MLZ)J-NSE-20140101},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29505043},
UT = {WOS:000429205700005},
doi = {10.1039/C7CP08027A},
url = {https://juser.fz-juelich.de/record/844929},
}