TY  - JOUR
AU  - Kolasinac, Rejhana
AU  - Kleusch, Christian
AU  - Braun, Tobias
AU  - Merkel, Rudolf
AU  - Csiszar, Agnes
TI  - Deciphering the Functional Composition of Fusogenic Liposomes
JO  - International journal of molecular sciences
VL  - 19
IS  - 2
SN  - 1422-0067
CY  - Basel
PB  - Molecular Diversity Preservation International
M1  - FZJ-2018-02326
SP  - 346 -
PY  - 2018
AB  - Cationic liposomes are frequently used as carrier particles for nucleic acid delivery. The most popular formulation is the equimolar mixture of two components, a cationic lipid and a neutral phosphoethanolamine. Its uptake pathway has been described as endocytosis. The presence of an aromatic molecule as a third component strongly influences the cellular uptake process and results in complete membrane fusion instead of endocytosis. Here, we systematically varied all three components of this lipid mixture and determined how efficiently the resulting particles fused with the plasma membrane of living mammalian cells. Our results show that an aromatic molecule and a cationic lipid component with conical molecular shape are essential for efficient fusion induction. While a neutral lipid is not mandatory, it can be used to control fusion efficiency and, in the most extreme case, to revert the uptake mechanism back to endocytosis
LB  - PUB:(DE-HGF)16
C6  - pmid:29364187
UR  - <Go to ISI:>//WOS:000427527400030
DO  - DOI:10.3390/ijms19020346
UR  - https://juser.fz-juelich.de/record/844995
ER  -