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@ARTICLE{GhoshMoulick:845260,
      author       = {Ghosh Moulick, Ranjita and Panaitov, Gregory and Du, Liping
                      and Mayer, Dirk and Offenhäusser, Andreas},
      title        = {{N}euronal adhesion and growth on nanopatterned
                      {EA}5-{POPC} synthetic membranes},
      journal      = {Nanoscale},
      volume       = {10},
      number       = {11},
      issn         = {2040-3372},
      address      = {Cambridge},
      publisher    = {RSC Publ.},
      reportid     = {FZJ-2018-02541},
      pages        = {5295 - 5301},
      year         = {2018},
      abstract     = {Biomimetic membranes create opportunities for various
                      applications, including the possibility of replacing
                      interacting cells in a cell–cell contact. Here we have
                      fractionated synthetic membranes using metal nano-grid
                      structures where EphrinA5 (EA5), a neuronal adhesion
                      promoter, was anchored via its Fc domain (immunoglobulin G
                      (IgG)-domain). FRAP experiments were performed to check the
                      confinement of the synthetic membrane within these
                      nano-structures. Rat cortical primary neurons were cultured
                      and live cell imaging techniques were used to monitor the
                      neuronal interaction with these fractionated synthetic
                      membranes. Computational imaging analysis of the
                      corresponding images elucidated interesting details of the
                      cellular behavior. The phenotypic cellular response on these
                      nano-membrane fractions was found to be similar to that on
                      non-fractionated synthetic membranes indicating that
                      although the number of focal adhesion points was low (due to
                      the reduced EA5 number) in the nano-sized membrane patches
                      perhaps some other factors like metal grid boundaries might
                      be playing a role in rendering the similarity.},
      cin          = {ICS-8},
      ddc          = {600},
      cid          = {I:(DE-Juel1)ICS-8-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:29498734},
      UT           = {WOS:000428787200033},
      doi          = {10.1039/C7NR08520F},
      url          = {https://juser.fz-juelich.de/record/845260},
}