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@TECHREPORT{Memmert:845543,
author = {Memmert, K.},
title = {{K}ontinuierliche {X}ylanaseproduktion mit {B}acillus
amyloliquefaciens},
volume = {2132},
number = {Juel-2132},
address = {Jülich},
publisher = {Kernforschungsanlage Jülich, Verlag},
reportid = {FZJ-2018-02770, Juel-2132},
series = {Berichte der Kernforschungsanlage Jülich},
pages = {VII, 169 p.},
year = {1987},
abstract = {Xylanase synthesis in$\textit{Bacillus}$
$\textit{amyloliquefaciens}$ DSM 7 was found to be
constitutive but controlled by catabolite repression. This
control mechanism caused a declining yield in xylanase
production, as soon as the culture reached a steady-state
growth in chemostate culture. It could be shown that the
catabolite repression of exoenzyme synthesis could be lifted
and xylanase production occured, when the bacteria were
subject to a defined oxygen limitation. This defined oxygen
limitation could be reached by holding the oxygen tension
inside narrow limits around 0.5\% of saturation. As this
range of low oxygen tensions only occured in transient
states but never in steady states of continuous cultivation,
it had to be fixed by varying the fermenter's stirrer speed
thus changing the oxygen transfer rate by an automatic
control. As a redox probe generates more accurate values at
this range of very low oxygen tensions than a
polarographical oxygenelectrode, the culture's redox
potential was used as the control signal. Comparative
measurements of redox potential and oxygen tension indicated
that the redox potential is proportional to the logarithm of
the oxygen tension. Holding the redox potential and oxygen
tension by control within this unstable non-steady range, a
constant high xylanase productivity of about 1000
U/(g$\cdot$h) could be obtained in continuous culture.},
cin = {PRE-2000},
cid = {I:(DE-Juel1)PRE2000-20140101},
pnm = {899 - ohne Topic (POF3-899)},
pid = {G:(DE-HGF)POF3-899},
typ = {PUB:(DE-HGF)3 / PUB:(DE-HGF)29},
url = {https://juser.fz-juelich.de/record/845543},
}