TY - JOUR AU - Schneider, Hannah AU - Wojciechowski, Tobias AU - Postma, Johannes Auke AU - Brown, Kathleen M AU - Lynch, Jonathan P TI - Ethylene modulates root cortical senescence in barley JO - Annals of botany VL - 122 IS - 1 SN - 1095-8290 CY - Oxford PB - Oxford University Press M1 - FZJ-2018-02883 SP - 95 - 105 PY - 2018 AB - Background and AimsRoot cortical senescence (RCS) is a poorly understood phenomenon with implications for adaptation to edaphic stress. It was hypothesized that RCS in barley (Hordeum vulgare L.) is (1) accelerated by exogenous ethylene exposure; (2) accompanied by differential expression of ethylene synthesis and signalling genes; and (3) associated with differential expression of programmed cell death (PCD) genes.MethodsGene expression of root segments from four barley genotypes with and without RCS was evaluated using quantitative real-time PCR (qRT-PCR). The progression of RCS was manipulated with root zone ethylene and ethylene inhibitor applications.Key ResultsThe results demonstrate that ethylene modulates RCS. Four genes related to ethylene synthesis and signalling were upregulated during RCS in optimal, low nitrogen and low phosphorus nutrient regimes. RCS was accelerated by root zone ethylene treatment, and this effect was reversed by an ethylene action inhibitor. Roots treated with exogenous ethylene had 35 and 46 % more cortical senescence compared with the control aeration treatment in seminal and nodal roots, respectively. RCS was correlated with expression of two genes related to programmed cell death (PCD).ConclusionsThe development of RCS is similar to root cortical aerenchyma formation with respect to ethylene modulation of the PCD process. LB - PUB:(DE-HGF)16 C6 - pmid:29897390 UR - <Go to ISI:>//WOS:000438280300010 DO - DOI:10.1093/aob/mcy059 UR - https://juser.fz-juelich.de/record/845671 ER -