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@ARTICLE{Beisel:8461,
      author       = {Beisel, K.G. and Jahnke, S. and Hofmann, D. and Köppchen,
                      S. and Schurr, U. and Matsubara, S.},
      title        = {{C}ontinuous {T}urnover of {C}arotenes and {C}hlorophyll a
                      in {M}ature {L}eaves of {A}rabidopsis {R}evealed by 14{CO}2
                      {P}ulse-{C}hase {L}abeling},
      journal      = {Plant physiology},
      volume       = {152},
      issn         = {0032-0889},
      address      = {Rockville, Md.: Soc.},
      publisher    = {JSTOR},
      reportid     = {PreJuSER-8461},
      pages        = {2188 -2199},
      year         = {2010},
      note         = {We thank Roberto Bassi and Luca Dall'Osto for kindly
                      providing the seeds of lut5 and lut2/npq2 mutants. Valuable
                      suggestions of Barry Osmond during the manuscript
                      preparation are much appreciated. K. G. B. acknowledges the
                      support for her Ph. D. thesis at the
                      Heinrich-Heine-Universitat Dusseldorf.},
      comment      = {Plant Physiology 2010 vol. 152 no. 4 2188-2199},
      booktitle     = {Plant Physiology 2010 vol. 152 no. 4
                       2188-2199},
      abstract     = {Carotenoid turnover was investigated in mature leaves of
                      Arabidopsis (Arabidopsis thaliana) by 14CO2 pulse-chase
                      labeling under control-light (CL; 130 micromol photons m(-2)
                      s(-1)) and high-light (HL; 1,000 micromol photons m(-2)
                      s(-1)) conditions. Following a 30-min 14CO2 administration,
                      photosynthetically fixed 14C was quickly incorporated in
                      beta-carotene (beta-C) and chlorophyll a (Chl a) in all
                      samples during a chase of up to 10 h. In contrast, 14C was
                      not detected in Chl b and xanthophylls, even when
                      steady-state amounts of the xanthophyll-cycle pigments and
                      lutein increased markedly, presumably by de novo synthesis,
                      in CL-grown plants under HL. Different light conditions
                      during the chase did not affect the 14C fractions
                      incorporated in beta-C and Chl a, whereas long-term HL
                      acclimation significantly enhanced 14C labeling of Chl a but
                      not beta-C. Consequently, the maximal 14C signal ratio
                      between beta-C and Chl a was much lower in HL-grown plants
                      (1:10) than in CL-grown plants (1:4). In lut5 mutants,
                      containing alpha-carotene (alpha-C) together with reduced
                      amounts of beta-C, remarkably high 14C labeling was found
                      for alpha-C while the labeling efficiency of Chl a was
                      similar to that of wild-type plants. The maximum 14C ratios
                      between carotenes and Chl a were 1:2 for alpha-C:Chl a and
                      1:5 for beta-C:Chl a in CL-grown lut5 plants, suggesting
                      high turnover of alpha-C. The data demonstrate continuous
                      synthesis and degradation of carotenes and Chl a in
                      photosynthesizing leaves and indicate distinct acclimatory
                      responses of their turnover to changing irradiance. In
                      addition, the results are discussed in the context of
                      photosystem II repair cycle and D1 protein turnover.},
      keywords     = {Arabidopsis: metabolism / Carbon Radioisotopes: metabolism
                      / Carotenoids: metabolism / Chlorophyll: metabolism / Plant
                      Leaves: metabolism / Carbon Radioisotopes (NLM Chemicals) /
                      Chlorophyll (NLM Chemicals) / Carotenoids (NLM Chemicals) /
                      chlorophyll a (NLM Chemicals) / J (WoSType)},
      cin          = {ICG-3 / ICG-4 / ZCH / JARA-ENERGY},
      ddc          = {580},
      cid          = {I:(DE-Juel1)ICG-3-20090406 / I:(DE-Juel1)VDB793 /
                      I:(DE-Juel1)ZCH-20090406 / $I:(DE-82)080011_20140620$},
      pnm          = {Terrestrische Umwelt},
      pid          = {G:(DE-Juel1)FUEK407},
      shelfmark    = {Plant Sciences},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:20118270},
      pmc          = {pmc:PMC2850008},
      UT           = {WOS:000276335900036},
      doi          = {10.1104/pp.109.151647},
      url          = {https://juser.fz-juelich.de/record/8461},
}