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@ARTICLE{Loomba:847885,
author = {Loomba, Varun and Huber, Gregor and von Lieres, Eric},
title = {{S}ingle-cell computational analysis of light harvesting in
a flat-panel photo-bioreactor},
journal = {Biotechnology for biofuels},
volume = {11},
number = {1},
issn = {1754-6834},
address = {London},
publisher = {BioMed Central},
reportid = {FZJ-2018-03211},
pages = {149},
year = {2018},
abstract = {BackgroundFlat-panel photo-bioreactors (PBRs) are
customarily applied for investigating growth of microalgae.
Optimal design and operation of such reactors is still a
challenge due to complex non-linear combinations of various
impact factors, particularly hydrodynamics, light
irradiation, and cell metabolism. A detailed analysis of
single-cell light reception can lead to novel insights into
the complex interactions of light exposure and algae
movement in the reactor.ResultsThe combined impacts of
hydrodynamics and light irradiation on algae cultivation in
a flat-panel PBR were studied by tracing the light exposure
of individual cells over time. Hydrodynamics and turbulent
mixing in this air-sparged bioreactor were simulated using
the Eulerian approach for the liquid phase and a slip model
for the gas phase velocity profiles. The liquid velocity was
then used for tracing single cells and their light exposure,
using light intensity profiles obtained from solving the
radiative transfer equation at different wavelengths. The
residence times of algae cells in defined dark and light
zones of the PBR were statistically analyzed for different
algal concentrations and sparging rates. The results
indicate poor mixing caused by the reactor design which can
be only partially improved by increased sparging
rates.ConclusionsThe results provide important information
for optimizing algal biomass productivity by improving
bioreactor design and operation and can further be utilized
for an in-depth analysis of algal growth by using advanced
models of cell metabolism.},
cin = {IBG-1 / IBG-2},
ddc = {570},
cid = {I:(DE-Juel1)IBG-1-20101118 / I:(DE-Juel1)IBG-2-20101118},
pnm = {583 - Innovative Synergisms (POF3-583)},
pid = {G:(DE-HGF)POF3-583},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29849766},
UT = {WOS:000433989900001},
doi = {10.1186/s13068-018-1147-3},
url = {https://juser.fz-juelich.de/record/847885},
}