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@INPROCEEDINGS{Longo:849968,
author = {Longo, Marialucia and Sebastiani, Federico and Paciaroni,
Alessandro and Orecchini, Andrea and Sacchetti, Francesco
and Petrillo, Caterina and Muthmann, Matthias and Texeira,
S. C. and de Francesco, A. and Comez, Lucia},
title = {{H}ydration-dependent dynamics of human telomeric
oligonucleotides investigated by inelastic neutron
scattering},
reportid = {FZJ-2018-04063},
year = {2017},
abstract = {The functionality of biomolecules, such as DNA, is related
not only to the structural but also to the dynamical
properties. The activation of fast structural fluctuations
on the pico- and nanosecond time windows enables slower
conformational rearrangements and provides a minimum degree
of flexibility required to carry out the biological
activity. Recently, the number of studies on guanine-rich
sequences at the end of telomeres is strongly increasing
because of their potential therapeutic target for cancer.
This kind of oligonucleotides can fold into a four-stranded
structure called G-quadruplex and then inhibit the activity
of telomerase, which is an enzyme responsible for the
immortalization of the cancer cells. In this context, an
inelastic neutron scattering study has been performed to
investigate how the hydration degree and the temperature
affect the thermal fluctuations of the intra-molecular
sequence d[AG3(AG3T2)3], which has been extensively used as
a model for human telomeres. A hydration-dependent dynamical
activation in weakly hydrated samples of oligonucleotides
was found showing a contribution from intra- and
inter-nucleotide dynamics.The trends of both amplitudes and
the characteristic timescales of the thermal fluctuations
point to a higher conformational flexibility of the human
telomeric sequence as compared to that of a long sequence of
DNA.},
month = {Jun},
date = {2017-06-27},
organization = {Neutrons for Health, Bad Reichenhall
(Germany), 27 Jun 2017 - 30 Jun 2017},
subtyp = {After Call},
cin = {JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
cid = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
I:(DE-Juel1)JCNS-1-20110106},
pnm = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
Neutron Research (JCNS) (POF3-623) / SINE2020 - World class
Science and Innovation with Neutrons in Europe 2020 –
SINE2020 (654000)},
pid = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4 /
G:(EU-Grant)654000},
experiment = {EXP:(DE-MLZ)SPHERES-20140101},
typ = {PUB:(DE-HGF)24},
url = {https://juser.fz-juelich.de/record/849968},
}