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@ARTICLE{Markov:849981,
author = {Markov, Aleksandr and Maybeck, Vanessa and Wolf, Nikolaus
and Mayer, Dirk and Offenhäusser, Andreas and Wördenweber,
Roger},
title = {{E}ngineering of {N}euron {G}rowth and {E}nhancing
{C}ell-{C}hip {C}ommunication via {M}ixed {SAM}s},
journal = {ACS applied materials $\&$ interfaces},
volume = {10},
number = {22},
issn = {1944-8252},
address = {Washington, DC},
publisher = {Soc.},
reportid = {FZJ-2018-04076},
pages = {18507 - 18514},
year = {2018},
abstract = {The interface between cells and inorganic surfaces
represents one of the key elements for bioelectronics
experiments and applications ranging from cell cultures and
bioelectronics devices to medial implants. In the present
paper, we describe a way to tailor the biocompatibility of
substrates in terms of cell growth and to significantly
improve cell-chip communication, and we also demonstrate the
reusability of the substrates for cell experiments. All
these improvements are achieved by coating the substrates or
chips with a self-assembled monolayer (SAM) consisting of a
mixture of organic molecules,
(3-aminopropyl)-triethoxysilane (APTES) and
(3-glycidyloxypropyl)-trimethoxysilane (GLYMO). By varying
the ratio of these molecules, we are able to tune the cell
density and live/dead ratios of rat cortical neurons
cultured directly on the mixed SAM as well as neurons
cultured on protein-coated SAMs. Furthermore, the use of the
SAM leads to a significant improvement in cell-chip
communications. Action potential signals of up to 9.4± 0.6
mV (signal-to-noise ratio up to 47) are obtained for HL-1
cells on microelectrode arrays. Finally, we demonstrate that
the SAMs facilitates a reusability of the samples for all
cell experiments with little re-processing.},
cin = {ICS-8},
ddc = {540},
cid = {I:(DE-Juel1)ICS-8-20110106},
pnm = {552 - Engineering Cell Function (POF3-552)},
pid = {G:(DE-HGF)POF3-552},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29763286},
UT = {WOS:000434895500010},
doi = {10.1021/acsami.8b02948},
url = {https://juser.fz-juelich.de/record/849981},
}