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@ARTICLE{Strehlau:851126,
author = {Strehlau, Jenny and Weber, Till and Lürenbaum, Constantin
and Bornhorst, Julia and Galla, Hans-Joachim and Schwerdtle,
Tanja and Winter, Martin and Nowak, Sascha},
title = {{T}owards quantification of toxicity of lithium ion battery
electrolytes - development and validation of a liquid-liquid
extraction {GC}-{MS} method for the determination of organic
carbonates in cell culture materials},
journal = {Analytical and bioanalytical chemistry},
volume = {409},
number = {26},
issn = {0016-1152},
address = {Berlin},
publisher = {Springer},
reportid = {FZJ-2018-04828},
pages = {6123 - 6131},
year = {2017},
abstract = {A novel method based on liquid-liquid extraction with
subsequent gas chromatography separation and mass
spectrometric detection (GC-MS) for the quantification of
organic carbonates in cell culture materials is presented.
Method parameters including the choice of extraction
solvent, of extraction method and of extraction time were
optimised and the method was validated. The setup allowed
for determination within a linear range of more than two
orders of magnitude. The limits of detection (LODs) were
between 0.0002 and 0.002 mmol/L and the repeatability
precisions were in the range of $1.5–12.9\%.$ It could be
shown that no matrix effects were present and recovery rates
between 98 and $104\%$ were achieved. The methodology was
applied to cell culture models incubated with commercial
lithium ion battery (LIB) electrolytes to gain more insight
into the potential toxic effects of these compounds. The
stability of the organic carbonates in cell culture medium
after incubation was studied. In a porcine model of the
blood-cerebrospinal fluid (CSF) barrier, it could be shown
that a transfer of organic carbonates into the brain facing
compartment took place.},
cin = {IEK-12},
ddc = {540},
cid = {I:(DE-Juel1)IEK-12-20141217},
pnm = {131 - Electrochemical Storage (POF3-131)},
pid = {G:(DE-HGF)POF3-131},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:28776071},
UT = {WOS:000411709000008},
doi = {10.1007/s00216-017-0549-6},
url = {https://juser.fz-juelich.de/record/851126},
}