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000851194 1001_ $$00000-0002-3289-7807$$aVay, Sabine Ulrike$$b0$$eCorresponding author
000851194 245__ $$aThe plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
000851194 260__ $$aLondon$$bBioMed Central$$c2018
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000851194 520__ $$aBackgroundMicroglia—the resident immune cells of the brain—are activated after brain lesions, e.g., cerebral ischemia, and polarize towards a classic “M1” pro-inflammatory or an alternative “M2” anti-inflammatory phenotype following characteristic temporo-spatial patterns, contributing either to secondary tissue damage or to regenerative responses. They closely interact with endogenous neural stem cells (NSCs) residing in distinct niches of the adult brain. The current study aimed at elucidating the dynamics of microglia polarization and their differential effects on NSC function.ResultsPrimary rat microglia in vitro were polarized towards a M1 phenotype by LPS, or to a M2 phenotype by IL4, while simultaneous exposure to LPS plus IL4 resulted in a hybrid phenotype expressing both M1- and M2-characteristic markers. M2 microglia migrated less but exhibit higher phagocytic activity than M1 microglia. Defined mediators switched microglia from one polarization state to the other, a process more effective when transforming M2 microglia towards M1 than vice versa. Polarized microglia had differential effects on the differentiation potential of NSCs in vitro and in vivo, with M1 microglia promoting astrocytogenesis, while M2 microglia supported neurogenesis. Regardless of their polarization, microglia inhibited NSC proliferation, increased NSC migration, and accelerated NSC differentiation.ConclusionOverall, this study shed light on the complex conditions governing microglia polarization and the effects of differentially polarized microglia on critical functions of NSCs in vitro and in vivo. Refining the understanding of microglia activation and their modulatory effects on NSCs is likely to facilitate the development of innovative therapeutic concepts supporting the innate regenerative capacity of the brain.
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000851194 7001_ $$0P:(DE-HGF)0$$aFlitsch, Lea Jessica$$b1
000851194 7001_ $$0P:(DE-HGF)0$$aRabenstein, Monika$$b2
000851194 7001_ $$0P:(DE-HGF)0$$aRogall, Rebecca$$b3
000851194 7001_ $$0P:(DE-HGF)0$$aBlaschke, Stefan$$b4
000851194 7001_ $$0P:(DE-HGF)0$$aKleinhaus, Judith$$b5
000851194 7001_ $$0P:(DE-HGF)0$$aReinert, Noémie$$b6
000851194 7001_ $$0P:(DE-HGF)0$$aBach, Annika$$b7
000851194 7001_ $$0P:(DE-Juel1)131720$$aFink, Gereon Rudolf$$b8
000851194 7001_ $$0P:(DE-HGF)0$$aSchroeter, Michael$$b9
000851194 7001_ $$0P:(DE-HGF)0$$aRueger, Maria Adele$$b10
000851194 773__ $$0PERI:(DE-600)2156455-3$$a10.1186/s12974-018-1261-y$$gVol. 15, no. 1, p. 226$$n1$$p226$$tJournal of neuroinflammation$$v15$$x1742-2094$$y2018
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