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@ARTICLE{Chcharo:851364,
      author       = {Chícharo, Alexandre and Martins, Marco and Barnsley,
                      Lester C. and Taouallah, Amal and Fernandes, João and
                      Silva, Bruno F. B. and Cardoso, Susana and Diéguez, Lorena
                      and Espiña, Begoña and Freitas, Paulo P.},
      title        = {{E}nhanced magnetic microcytometer with 3{D} flow focusing
                      for cell enumeration},
      journal      = {Lab on a chip},
      volume       = {18},
      number       = {17},
      issn         = {1473-0189},
      address      = {Cambridge},
      publisher    = {RSC},
      reportid     = {FZJ-2018-05049},
      pages        = {2593 - 2603},
      year         = {2018},
      abstract     = {We report the design and characterization of a lateral and
                      vertical hydrodynamic focusing feature for whole cell
                      detection on a miniaturized flow cytometer. The developed
                      system, based on magnetic sensing, incorporates spin valve
                      sensors on the bottom of the microfluidic channels that
                      detect cells labeled with magnetic beads. An adaptable 3D
                      hydrodynamic focusing system was developed that pushes
                      labeled cells towards the bottom of the microchannel, closer
                      to the sensors, allowing increased signal amplitude for
                      cells labeled with magnetic beads and enhanced
                      discrimination of labeled cells. Fluorescence microscopy
                      indicates that the lateral and vertical hydrodynamic
                      focusing effect was adequately implemented, consistent with
                      simulation predictions. The sensitivity of the system to
                      detect labeled cells was improved by at least two-fold. By
                      estimating the coverage of magnetic beads on cells, the
                      signal from labeled cells could be predicted using a
                      mathematical model, which also demonstrated the sensitivity
                      of the signal to the height of the cells relative to the
                      sensor. The system is versatile allowing interchangeable
                      flow rates for cells with different diameters.},
      cin          = {JCNS-FRM-II / Neutronenstreuung ; JCNS-1},
      ddc          = {004},
      cid          = {I:(DE-Juel1)JCNS-FRM-II-20110218 /
                      I:(DE-Juel1)JCNS-1-20110106},
      pnm          = {6G15 - FRM II / MLZ (POF3-6G15) / 6G4 - Jülich Centre for
                      Neutron Research (JCNS) (POF3-623)},
      pid          = {G:(DE-HGF)POF3-6G15 / G:(DE-HGF)POF3-6G4},
      experiment   = {EXP:(DE-MLZ)NOSPEC-20140101},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30027969},
      UT           = {WOS:000443279400009},
      doi          = {10.1039/C8LC00486B},
      url          = {https://juser.fz-juelich.de/record/851364},
}