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@ARTICLE{Hoffmann:851664,
      author       = {Hoffmann, Marco and Hersch, Nils and Merkel, Rudolf and
                      Csiszar, Agnes and Hoffmann, Bernd},
      title        = {{C}hanging the {W}ay of {E}ntrance: {H}ighly {E}fficient
                      {T}ransfer of m{RNA} and si{RNA} via {F}usogenic
                      {N}ano-{C}arriers},
      journal      = {Journal of biomedical nanotechnology},
      volume       = {15},
      number       = {1},
      issn         = {1550-7033},
      address      = {Valencia, Calif.},
      publisher    = {American Scientific Publ.},
      reportid     = {FZJ-2018-05214},
      pages        = {170 - 183},
      year         = {2019},
      abstract     = {Transferring nucleic acids into mammalian cells heavily
                      influences life science for decades. While first
                      applications mainly dealt with DNA transfer for various
                      purposes as e.g., plasmid encoded protein expression or
                      generation of mutant strains, subsequent applications
                      additionally transferred RNA molecules of mainly small
                      lengths for specific knockdown (RNAi) or site-specific
                      genome modification (gRNA). Significant improvements in full
                      length mRNA generation and extension of mRNA lifetimes
                      additionally allows their use for transient expression in
                      latest times. For all of these types of nucleic acids the
                      most common cell incorporation method is based on
                      complexation and subsequent endosomal uptake. This so-called
                      lipofection can be used theoretically for almost any
                      mammalian cell type and a tremendous number of different
                      product compositions exist in order to deal with drawbacks
                      as transfer efficiency, cell type selectivity, endosomal
                      degradation, slow uptake and cytotoxicity. In contrast, new
                      methods transfer complexed RNA molecules directly into the
                      cytoplasm using liposomal nano-carriers that fuse with
                      cellular plasma membranes immediately upon contact to free
                      functional nucleic acids directly into the cytoplasm. Here,
                      we compare both methods in detail with special focus on
                      robustness, short- and long-term cytotoxicity, efficiency
                      and functionality for various types of transferred RNA. Our
                      data clearly indicate that direct RNA incorporation via
                      fusogenic nano-carriers circumvents most endosomal
                      uptake-based challenges, making it to a most promising
                      alternative for nucleic acid transfer.},
      cin          = {ICS-7},
      ddc          = {540},
      cid          = {I:(DE-Juel1)ICS-7-20110106},
      pnm          = {552 - Engineering Cell Function (POF3-552)},
      pid          = {G:(DE-HGF)POF3-552},
      typ          = {PUB:(DE-HGF)16},
      pubmed       = {pmid:30480524},
      UT           = {WOS:000451075000013},
      doi          = {10.1166/jbn.2019.2663},
      url          = {https://juser.fz-juelich.de/record/851664},
}