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@ARTICLE{Yang:856019,
author = {Yang, Bo and Voiniciuc, Cătălin and Fu, Lanbao and
Dieluweit, Sabine and Klose, Holger and Usadel, Björn},
title = {{TRM}4 is essential for cellulose deposition in
{A}rabidopsis seed mucilage by maintaining cortical
microtubule organization and interacting with {CESA}3552},
journal = {The new phytologist},
volume = {221},
number = {2},
issn = {0028-646X},
address = {Oxford [u.a.]},
publisher = {Wiley-Blackwell},
reportid = {FZJ-2018-05704},
pages = {881-895},
year = {2019},
abstract = {The differentiation of the seed coat epidermal (SCE) cells
in Arabidopsis thaliana leads to the production of a large
amount of pectin‐rich mucilage and a thick cellulosic
secondary cell wall. The mechanisms by which cortical
microtubules are involved in the formation of these
pectinaceous and cellulosic cell walls are still largely
unknown. Using a reverse genetic approach, we found that
TONNEAU1 (TON1) recruiting motif 4 (TRM4) is implicated in
cortical microtubule organization in SCE cells, and
functions as a novel player in the establishment of mucilage
structure. TRM4 is preferentially accumulated in the SCE
cells at the stage of mucilage biosynthesis. The loss of
TRM4 results in compact seed mucilage capsules, aberrant
mucilage cellulosic structure, short cellulosic rays and
disorganized cellulose microfibrils in mucilage. The defects
could be rescued by transgene complementation of trm4
alleles. Probably, this is a consequence of a disrupted
organization of cortical microtubules, observed using
fluorescently tagged tubulin proteins in trm4 SCE cells.
Furthermore, TRM4 proteins co‐aligned with microtubules
and interacted directly with CELLULOSE SYNTHASE 3 in two
independent assays. Together, the results indicate that TRM4
is essential for microtubule array organization and
therefore correct cellulose orientation in the SCE cells, as
well as the establishment of the subsequent mucilage
architecture.},
cin = {ICS-7 / IBG-2},
ddc = {580},
cid = {I:(DE-Juel1)ICS-7-20110106 / I:(DE-Juel1)IBG-2-20101118},
pnm = {552 - Engineering Cell Function (POF3-552) / 582 - Plant
Science (POF3-582) / 583 - Innovative Synergisms (POF3-583)},
pid = {G:(DE-HGF)POF3-552 / G:(DE-HGF)POF3-582 /
G:(DE-HGF)POF3-583},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:30277578},
UT = {WOS:000453883200030},
doi = {10.1111/nph.15442},
url = {https://juser.fz-juelich.de/record/856019},
}