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@ARTICLE{Zaffagnini:856514,
author = {Zaffagnini, G. and Savova, A. and Danieli, A. and Romanov,
J. and Tremel, S. and Ebner, M. and Peterbauer, T. and
Sztacho, M. and Trapannone, R. and Tarafder, A. K. and
Sachse, Carsten and Martens, S.},
title = {{P}hasing out the bad-{H}ow {SQSTM}1/p62 sequesters
ubiquitinated proteins for degradation by autophagy},
journal = {Autophagy},
volume = {14},
number = {7},
issn = {1554-8627},
address = {Abingdon, Oxon},
publisher = {Taylor $\&$ Francis},
reportid = {FZJ-2018-05901},
pages = {1280-1282},
year = {2018},
abstract = {The degradation of misfolded, ubiquitinated proteins is
essential for cellular homeostasis. These proteins are
primarily degraded by the ubiquitin-proteasome system (UPS)
and macroautophagy/autophagy serves as a backup mechanism
when the UPS is overloaded. How autophagy and the UPS are
coordinated is not fully understood. During the autophagy of
misfolded, ubiquitinated proteins, referred to as
aggrephagy, substrate proteins are clustered into larger
structures in a SQSTM1/p62-dependent manner before they are
sequestered by phagophores, the precursors to
autophagosomes. We have recently shown that SQSTM1/p62 and
ubiquitinated proteins spontaneously phase separate into
micrometer-sized clusters in vitro. This enabled us to
characterize the properties of the ubiquitin-positive
substrates that are necessary for the SQSTM1/p62-mediated
cluster formation. Our results suggest that aggrephagy is
triggered by the accumulation of substrates with multiple
ubiquitin chains and that the process can be inhibited by
active proteasomes.},
cin = {ER-C-3},
ddc = {570},
cid = {I:(DE-Juel1)ER-C-3-20170113},
pnm = {551 - Functional Macromolecules and Complexes (POF3-551)},
pid = {G:(DE-HGF)POF3-551},
typ = {PUB:(DE-HGF)16},
pubmed = {pmid:29929426},
UT = {WOS:000441624100020},
doi = {10.1080/15548627.2018.1462079},
url = {https://juser.fz-juelich.de/record/856514},
}